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Fluorescent labeling and tracing of immobilized efficient degrading bacterium DNB-S1 and its remediation efficiency of DBP contaminated soil

文献类型： 外文期刊

作者： Wang, Lei 1 ; Jia, Xiaochen 1 ; Dou, Zeyu 1 ; Li, Xiaoqian 1 ; Bao, Wenjing 1 ; Ma, Chaoran 1 ; Wang, Hongye 1 ; Dong, Maofeng 2 ; Zhang, Ying 1 ;

作者机构： 1.Northeast Agr Univ, Sch Resources & Environm, Harbin 150030, Peoples R China

2.Shanghai Acad Agr Sci, Pesticide Safety Evaluat Res Ctr, 2901 Beizhai Rd, Shanghai, Peoples R China

关键词： GFP fluorescence Labeling; DBP; Immobilized bacteria; Biodegradation; Dynamics

期刊名称：CHEMOSPHERE （ 影响因子：8.8； 五年影响因子：8.3 ）

ISSN： 0045-6535

年卷期： 2023 年 320 卷

页码：

收录情况： SCI

摘要： Dibutyl phthalate (DBP) is an organic pollutant frequently detected in soil, and is a reproductive poison that harms animals both before and after birth and has mutagenic, teratogenic, and carcinogenic effects. DBP removal from farmland has been the subject of extensive research in recent years. Efficient DBP degrading bacterial strains were screened in the laboratory. GFP (Green fluorescent protein) labeled degradation strain GFP-DNB-S1 was analyzed for its activity and dynamics. Using sodium alginate (SA) and nano-hydroxyapatite (n-HAP) as carrier materials and CaCl2 as a cross-linking agent, the immobilized microbial agent n-HAP/SA + DNB-S1 was prepared by embedding cross-linking immobilization technology to study the remediation effect of DBP contaminated soil. The best formation effect of immobilized materials (n-HAP/SA) was found when the SA to n -HAP ratio was 3:2. When compared to single SA immobilized bacteria, n-HAP/SA immobilized bacteria improved the surface roughness and porosity of the microspheres. After 70 days, LED light revealed that the immobilized bacteria's GFP green fluorescent protein expression was stable. At 70 days, the initial DBP concentration of 500 mg center dot L-1 degraded at a rate of 69.9%. The degrading bacteria had no effect on DBP degradation before and after being labeled with GFP. The n-HAP/SA immobilized bacteria offered a better living environment for microor-ganisms due to their rougher surface and a greater number of pores. This protected the microorganisms and increased the efficiency of DBP degradation. When the concentration of DBP in contaminated soil was set to 20 mg center dot kg-1 and the n-HAP/SA + DNB-S1 immobilized bacterial agent was applied to the soil, the rate of DBP degradation was determined to be 93.34%. The degradation process followed First-order degradation kinetics, which improved the physical and chemical properties of the soil as well as its fertility.

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