您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization and comparative transcriptome analyses of Salmonella enterica Enteritidis strains possessing different chlorine tolerance profiles

文献类型： 外文期刊

作者： Wang, Wensi 1 ; Xiao, Xingning 2 ; Wang, Hongxun 3 ; Wang, Sheng 2 ; Xiao, Yingping 2 ; Yang, Hua 2 ; Hou, Wenfu 1 ; Wang, Wen 2 ;

作者机构： 1.Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Lab Qual & Safety Risk Assessment Agroprod Hangzh, MOA,Inst Agroprod Safety & Nutr, Hangzhou, Zhejiang, Peoples R China

3.Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China

4.Ningbo Univ, Coll Food & Pharmaceut Sci, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo, Zhejiang, Peoples R China

关键词： Chlorine tolerance; Transcriptome sequencing; Oxidative stress response; Efflux pump; Bacterial microcompartments

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.056； 五年影响因子：6.295 ）

ISSN： 0023-6438

年卷期： 2022 年 169 卷

页码：

收录情况： SCI

摘要： We compared viability, morphology, enzyme activity and gene expression patterns between the chlorine tolerant S. Enteritidis RE 13 and the sensitive strain S. Enteritidis CVCC1806. As expected, viability of strain CVCC1806 was significantly lower than for RE 13 after exposure to 100 mg/L NaClO for 20 min and the sensitive strain displayed severe cell membrane damage and intracellular ATP depletion compared with the tolerant strain. The NAD+/NADH ratios for the sensitive vs tolerant strain were also significantly (p < 0.05) different (3.59 +/- 0.95 vs. 0.88 +/- 0.47) and indicated that tolerance included adaptation to redox perturbations. Transcriptomes of these two strains following chlorine exposure identified significant up-regulation of efflux pump systems (proV/W, OmpF, marA), oxidative stress response (trxC, yhhP, soxS) and DNA repair (dnaQ, polB, yaiV) for the tolerant RE 13 strain. These data indicated that S. Enteritidis RE 13 has a more robust response to oxidative stress that acted to maintain its intracellular redox balance to adapt and survive in the presence of chlorine. Noteworthy, the operon gene cluster of the bacterial microcompartment PDU were up-regulated about 64-fold in tolerant strain RE 13 compared with the sensitive strain, posing a more serious threat to human intestinal health.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台