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Prevalence, toxin-typing and antimicrobial susceptibility of Clostridium perfringens in sheep with different feeding modes from Gansu and Qinghai provinces, China

文献类型： 外文期刊

作者： Wu, Ke 1 ; Feng, Hang 1 ; Ma, Jiangang 2 ; Wang, Bin 1 ; Feng, Jie 1 ; Zhang, Hui 3 ; Jiang, Yanfen 1 ; Li, Ruichao 4 ; Wang, Juan 1 ; Yang, Zengqi 1 ;

作者机构： 1.Northwest A&F Univ, Coll Vet Med, Yangling, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Zhejiang, Peoples R China

3.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base MOST, Nanjing 210014, Peoples R China

4.Yangzhou Univ, Coll Vet Med, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China; Zhejiang Acad Agr Sci, Inst Agro Prod Safety & Nutr, Hangzhou, Zhejiang, Peoples R China

关键词： Clostridium perfringens; antimicrobial resistance; Sheep; Tibetan sheep; Whole genome sequencing

期刊名称：ANAEROBE （ 影响因子：2.837； 五年影响因子：3.351 ）

ISSN： 1075-9964

年卷期： 2022 年 73 卷

页码：

收录情况： SCI

摘要： Objective: The purpose of this study was to determine the prevalence and antimicrobial resistance of Clostridium perfringens from sheep (intensive husbandry) in Gansu and Tibetan sheep (extensive husbandry) in Qinghai, China. Methods: 400 fecal samples (sheep, n 1/4 320, Tibetan sheep, n 1/4 80) were collected from Gansu and Qinghai for C. perfringens isolation. Toxin genes were detected by PCR, antimicrobial susceptibility testing was carried out by broth microdilution method, and whole genome was sequenced using Illumina HiSeq. Results: 83 strains of C. perfringens (sheep, n 1/4 47; Tibetan sheep, n 1/4 36) were isolated from the samples. 44.5% (37/83) of the isolates were positive for cpb2, while 34.9% (29/83) of the isolates were positive for cna. 95.2% isolates were resistant to sulfonamides, followed by tetracycline (22.9%), ampicillin (14.5%), penicillin (10.8%), doxycycline (4.8%), and amoxicillin (1.2%). The isolates from same source shared similar allelic profile and closer genetic relationship. A total of 14 toxin genes and 11 antimicrobial resistance genes were detected among the sequenced isolates, and 10 sequenced C. perfringens isolates carried multiple (n similar to 3) antimicrobial-resistance genes. Moreover, oxazolidinone-resistant gene optrA was detected in one isolate from Tibetan sheep, which co-harbored tetA(P), aac(60)-aph(200), ant(6)-Ib, erm(Q), fexA, tet(44), erm(A) and erm(B). Conclusions: C. perfringens from sheep and Tibetan sheep shared different prevalence rates and antimicrobial-resistance, and the isolates from same source shared closer genetic relationship. (c) 2022 Elsevier Ltd. All rights reserved.

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