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Simultaneous preparation of edible quality medium and high purity diacylglycerol by a novel combined approach

文献类型： 外文期刊

作者： Li, Daoming 1 ; Zhong, Xiaorong 1 ; Faiza, Muniba 2 ; Wang, Weifei 3 ; Lian, Weishuai 4 ; Liu, Ning 1 ; Wang, Yonghua 5 ;

作者机构： 1.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China

2.Jamia Millia Islamia, Dept Biotechnol, New Delhi 110025, India

3.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Peoples R China

4.Henan Univ Anim & Husb & Econ, Dept Food & Biol Engn, Zhengzhou 450046, Peoples R China

5.South China Univ Technol, Guangdong Res Ctr Lipid Sci & Appl Engn Technol, Sch Food Sci & Engn, Guangzhou 510640, Peoples R China

关键词： Diacylglycerol; Partial hydrolysis; Partial glyceride lipase; Esterification; Edible quality

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）

ISSN： 0023-6438

年卷期： 2021 年 150 卷

页码：

收录情况： SCI

摘要： Diacylglycerol (DAG) is functional oil with many beneficial effects. However, the current approaches for DAG preparation face the problems of the low utilization rate of substrates and producing large amounts of by-product. Here, we report a novel approach for simultaneous preparation of edible quality medium and high purity DAG by combining enzymatic partial hydrolysis and a novel partial glyceride lipase (PGL)-catalyzed esterification. Firstly, four lipases were screened for their capability in DAG preparation by partial hydrolysis and Lipase DF "Amano" 15 was the best. Under the optimal conditions, scale-up reaction was performed and residues containing 55.2% DAG were obtained after molecular distillation. Subsequently, the collected distillates were used as substrate for DAG synthesis by a novel PGL-catalyzed esterification and PGL AOL-V269D was selected. Under the optimized conditions, scale-up reaction was performed and residues containing 99.3% DAG were obtained after molecular distillation. The collected distillates were recycled for further DAG synthesis. Finally, the obtained medium and high purity DAG were characterized, lacking glycidyl esters and 3-chloro-1,2-propane-diol esters, and with low acid and peroxide value reaching the edible quality. Overall, the proposed novel procedure enabled the substrates to be used extensively without by-product production.

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