Biodegradable Imiquimod-Loaded Mesoporous Organosilica as a Nanocarrier and Adjuvant for Enhanced and Prolonged Immunity against Foot-and-Mouth Disease Virus in Mice
文献类型： 外文期刊
作者： Yin, Wenzhu 1 ; Xuan, Dechun 1 ; Wang, Haiyan 1 ; Zhou, Mingxu 1 ; Deng, Bihua 1 ; Ma, Fang 1 ; Lu, Yu 1 ; Zhang, Jinqiu 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Minist Sci & Technol,State Key Lab Cultivat Base, Inst Vet Immunol & Engn,Jiangsu Key Lab Food Qual, Nanjing 210014, Peoples R China
2.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
3.Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Jiangsu, Peoples R China
关键词： mesoporous organosilica nanoparticles; imiquimod; FMDV; adjuvant; immunity
期刊名称：ACS APPLIED BIO MATERIALS （ 影响因子：4.7； 五年影响因子：4.6 ）
ISSN： 2576-6422
年卷期： 2022 年 5 卷 6 期
页码：
收录情况： SCI
摘要： Foot-and-mouth disease (FMD), a serious, fast-spreading, and virulent disease, has led to huge economic losses to people all over the world. Vaccines are the most effective way to control FMD. However, the weak immunogenicity of inactivated FMD virus (FMDV) requires the addition of adjuvants to enhance the immune effectiveness of the vaccines. Herein, we formulated and fabricated biodegradable dendritic mesoporous tetrasulfide-doped organosilica nanoparticles SOMSN with imiquimod complex (SOMSN-IMQ) and used it as a platform for FMD vaccine delivery and as an adjuvant. SOMSN-IMQ demonstrated excellent stability for 6 months when stored in PBS, while it could be completely degraded within 42 days in SBF at room temperature. Biosafety experiments such as cell toxicity, hemolysis, and histology indicated that the asprepared SOMSN-IMQ showed nontoxicity and good biocompatibility. Furthermore, SOMSN-IMQ exhibited a maximum adsorption capacity of 1000 mu g/mg for inactivated FMDV antigens. Our results showed that SOMSN-IMQ can be effectively engulfed by RAW264.7 cells in a dose-dependent manner. After immunization, SOMSN-IMQ@FMDV can elicit persistent higher antibody levels, higher IgG2a/IgG1 ratio, and cytokine expression, which indicated that SOMSN-IMQ@FMDV triggered superior humoral and cellular immune responses. Moreover, SOMSN-IMQ could provoke maturation and activation of dendritic cells in lymph nodes (LDCs) as well as the proliferation of lymphocytes in vivo. Thus, SOMSN-IMQ could promote effective and potent immunity and provide a promising adjuvant platform for FMDV vaccination with acceptable safety.
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