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Molecular cloning and expression analysis of two beta-defensin genes in the blunt snout bream (Megalobrama amblycephala)

文献类型: 外文期刊

作者: Liang, Tao 1 ; Wang, Dan-Dan 1 ; Zhang, Gui-Rong 1 ; Wei, Kai-Jian 1 ; Wang, Wei-Min 1 ; Zou, Gui-Wei 3 ;

作者机构: 1.Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China

2.Freshwater Aquaculture Collaborat Ctr Hubei Prov, Wuhan 430070, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr, Wuhan 430223, Peoples R Chin

关键词: Antimicrobial peptide;beta-Defensins;Megalobrama amblycephala;Gene expression;Bacterial stimulation

期刊名称:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY ( 影响因子:2.231; 五年影响因子:2.215 )

ISSN: 1096-4959

年卷期: 2013 年 166 卷 1 期

页码:

收录情况: SCI

摘要: beta-Defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. In this study, the part-length cDNA sequences of two beta-defensin genes (maBD-1, maBD-2) in blunt snout bream (Megalobrama amblycephala) were identified. Homology analysis showed that the cDNA sequences of maBD-1 and maBD-2 had high similarities to those in common carp and zebrafish. Real-time quantitative PCR results exhibited that expression level of maBD-1 in juvenile tissues was the highest in skin, followed by blood and liver, whereas maBD-2 was lowly expressed in liver, kidney, brain and foregut In the early development period, fertilized eggs to 31-day post-hatching (dph) larvae, the expression levels of maBD-1 were higher at the stage from heart beat stage to 3 dph with the highest value at 1 dph, whereas maBD-2 was expressed higher at fertilized eggs and late cleavage stages. Following bacterial stimulation in vivo by Aeromonas sobria, maBD-2 expressions were significantly up-regulated in liver, skin, gill, and foregut of juveniles, and maBD-1 expressions were significantly up-regulated in liver and skin. The results suggest that maBD-1 and maBD-2 may play important roles in protecting blunt snout bream embryos, fry and juveniles from pathogenic microbe invading. (C) 2013 Elsevier Inc All rights reserved.

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