文献类型： 外文期刊

作者： Hu, Yuan-Hong ¹ ; Zhou, Xu ¹ ; Jiang, Xin-Xin ¹ ; Zhang, Gui-Rong ¹ ; Shi, Ze-Chao ³ ; Ji, Wei ¹ ; Ma, Xu-Fa ¹ ; Luo, Zun ¹ ;

作者机构： 1.Huazhong Agr Univ, Natl Demonstrat Ctr Expt Aquaculture Educ, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Minist Agr & Rural Affairs, Coll Fisheries, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs, Wuhan 430223, Peoples R China

4.Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China

关键词： Pelteobagrus fulvidraco; Pf_TNF-alpha; Pf_TNFR1; Pf_TNFR2; Gene expression; Functional analysis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 185 卷

页码：

收录情况： SCI

摘要： Inflammation is a common manifestation of body immunity and mediates a cascade of cytokines. Tumor necrosis factor-a (TNF-alpha), as a multi-effect cytokine, plays an important role in the inflammatory response by interacting with its receptor (TNFR). In this study, Pf TNF-alpha, Pf TNFR1 and Pf TNFR2 genes were cloned from yellow catfish (Pelteobagrus fulvidraco), and bioinformatics analyses showed that the three genes were conserved and possessed similar sequence characteristics as those of other vertebrates. The qPCR results showed that Pf TNF-alpha, Pf TNFR1 and Pf TNFR2 mRNAs were constitutively expressed in 14 tissues and the lymphocytes of four tissues from healthy adults. The mRNA expression levels of Pf TNF-alpha and Pf TNFR1 genes were significantly up-regulated in the spleen, liver, trunk kidney, head kidney and gill after Edwardsiella ictaluri infection, while the mRNA expression of Pf TNFR2 was significantly up-regulated in the spleen, and down-regulated in the liver and gill. In the isolated peripheral blood leukocytes (PBLs) of yellow catfish, the expression of Pf TNF-alpha mRNA was notably up-regulated and the two Pf TNFR transcripts were distinctly down-regulated after stimulation with lipopolysaccharides (LPS), peptidoglycan (PGN), polyinosinic-polycytidylic acid (Poly I:C) and phytohaemagglutinin (PHA). After stimulated by recombinant (r) Pf_sTNF protein, the mRNA expressions of various inflammatory factors genes were up-regulated in the PBLs. Meanwhile, rPf_sTNF promoted the phagocytic activity of leukocytes, whereas the activity mediated by rPf sTNF could be inhibited by rPf TNFR1CRD2/3 and rPf TNFR2CRD2/ 3. The up-regulation of TNF-alpha and IL-1 beta mRNAs expression triggered by rPf_sTNF could be inhibited by MAPK inhibitor (VX-702) and NF-kappa B inhibitor (PDTC). rPf_sTNF induced the expression of FADD mRNA in PBLs and increased the apoptotic rate of PBLs, and inhibiting the NF-kappa B and MAPK signal pathways could enhance the apoptosis of PBLs. The results indicate that Pf TNF-alpha, Pf TNFR1 and Pf TNFR2 play important roles in the immune response of yellow catfish to bacterial invasion.