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Bioinformatics characteristics and expression analysis of IL-8 and IL-10 in largemouth bass ( Micropterus salmoides ) upon Nocardia seriolae infection

文献类型： 外文期刊

作者： Wang, Qin 1 ; Zhao, Zhangchun 1 ; Sun, Ruhan 1 ; Shi, Zechao 2 ; Zhang, Yaqian 1 ; Wang, Bingchao 1 ; Zhang, Xuezhen 1 ; Ji, Wei 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries,Key Lab Agr Anim Genet Breeding & R, Dept Aquat Anim Med,Minist Agr & Rural Affair, Key Lab Freshwater Anim Breeding,Minist Educ, Wuhan 430070, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

关键词： Largemouth bass(Micropterussalmoides); IL-8; IL-10; Inflammatory response; Nocardia seriolae

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 148 卷

页码：

收录情况： SCI

摘要： IL -8 and IL -10 are crucial inflammatory cytokines that participate in defending host cells against infections. To demonstrate the function of the two interleukin genes in largemouth bass ( Micropterus salmoides ), we initially cloned and identified the cDNA sequences of il -8 and il -10 in largemouth bass, referred to as Msil-8 and Msil-10 , respectively. The open reading frame (ORF) of Msil-8 was 324 bp in length, encoding 107 amino acids, while the ORF of Msil-10 consisted of 726 bp and encoded 241 amino acids. Furthermore, the functional domains of the SCY domain in Ms IL-8 and the IL -10 family signature motif in Ms IL-10 were highly conserved across vertebrates. Additionally, both Ms IL-8 and Ms IL-10 showed close relationships with M. dolomieu . Constitutive expression of Msil-8 and Msil-10 was observed in various tissues, with the highest level found in the head kidney. Subsequently, largemouth bass were infected with Nocardia seriolae via intraperitoneal injection to gain a further understanding of the function of these two genes. Bacterial loads were initially detected in the foregut, followed by the midgut, hindgut, and liver. The mRNA expression of Msil-8 was significantly down-regulated after infection, especially at 2 days post -infection (DPI), with a similar expression to Msil-10 . In contrast, the expression of Msil-8 and Msil-10 was significantly upregulated in the foregut at 14 DPI. Taken together, these results reveal that the function of IL8 and IL -10 was likely hindered by N. seriolae , which promoted bacterial proliferation and intercellular diffusion.

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