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Molecular characterization and expression analyses of five genes involved in the MyD88-dependent pathway of yellow catfish (Pelteobagrus fulvidraco) responding to challenge of Aeromonas hydrophila

文献类型： 外文期刊

作者： Yuan, Yujie 1 ; Shi, Zechao 2 ; Wang, Qin 1 ; Guo, Mengge 1 ; Yuan, Le 3 ; Zhao, Zhangchun 1 ; Liu, Sixue 1 ; Wu, Chen 1 ; Sun, Ruhan 1 ; Wang, Bingchao 1 ; Ouyang, Gang 3 ; Ji, Wei 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Dept Aquat Anim Med, Key Lab Freshwater Anim Breeding,Key Lab Agr Anim, Wuhan 430070, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

4.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

关键词： Pelteobagrus fulvidraco; MyD88; IRAK4; IRAK1; TRAF6; NF kappa B1; Gene expression; Aeromonas hydrophila

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2023 年 137 卷

页码：

收录情况： SCI

摘要： MyD88-dependent pathway mediated by Toll-like receptor is one of the vital ways activating immune responses. In order to identify the role of MyD88-dependent signaling pathway in yellow catfish, the Pf_MyD88, Pf_IRAK4, Pf_IRAK1, Pf_TRAF6 and Pf_NF kappa B1 (p105) (Pf: abbreviation of Pelteobagrus fulvidraco) were cloned and characterized respectively. The Pf_MyD88, Pf_IRAK4, Pf_IRAK1 and Pf_TRAF6 were all highly conserved among species and showed the highest homology to that of Pangasianodon hypophthalmus. Pf_NF kappa B1 showed the highest homology to that of Ictalurus punetaus. All of the five genes showed similar expression patterns in various tissues, with the highest expression level in the liver. These genes also showed similar expression levels in different embryonic development stages, except Pf_IRAK4. The higher expression level was detected from fertilized eggs to 1 day post hatching (dph), lower expression from 3 dph to 30 dph. After stimulation of inactivated Aeromonas hydrophila, the mRNA expressions of Pf_MyD88, Pf_IRAK4, Pf_IRAK1, Pf_TRAF6 and Pf_NF kappa B1 were significantly increased at 24 h in the liver, spleen, head kidney and trunk kidney, suggesting that all the five genes were involved in the innate immune response of yellow catfish. These results showed that MyD88-dependent signaling pathway plays important roles for disease defensing in the innate immune response. Meanwhile, inactivated A. hydrophila can cause strong innate immune response, which provides theoretical bases for the application of inactivated vaccines in defense against bacterial diseases of teleost.

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