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Bioinformatics characteristics and expression analysis of TLR3 and its adaptor protein TRIF in largemouth bass (Micropterus salmoides) upon Flavobacterium columnare infection

文献类型： 外文期刊

作者： Zhao, Zhangchun 1 ; Liu, Sixue 1 ; Wu, Chen 1 ; Wang, Qin 1 ; Zhang, Yaqian 1 ; Wang, Bingchao 1 ; Wang, Long 2 ; Sun, Ruhan 1 ; Guo, Mengge 1 ; Ji, Wei 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries,Minist Educ, Dept Aquat Anim Med,Key Lab Agr Anim Genet Breedin, Key Lab Freshwater Anim Breeding,Minist Agr & Rura, Wuhan 430070, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

关键词： Micropterus salmoides; TLR3; TRIF; Gene expression; Flavobacterium columnare

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2023 年 872 卷

页码：

收录情况： SCI

摘要： TLR3 and TRIF (adaptor protein for TLR3) are vital to the MyD88-independent pathway mediated by Toll-like receptors (TLRs). In order to identify the role of TLR3 and TRIF in Micropterus salmoides, the Ms_TLR3 and Ms_TRIF (Ms: abbreviation for M. salmoides) were cloned and characterized in this study. The open reading frames (ORFs) of Ms_TLR3 and Ms_TRIF genes were 2736 bp and 1791 bp in length, encoding 911 and 596 amino acids, respectively. The protein structure of Ms_TLR3 includes a signal peptide, 18 LRR-related domains, a low complexity region, a transmembrane region, and a TIR domain. However, only a TIR domain and a coiled coil domain were found in Ms_TRIF. Both Ms_TLR3 and Ms_TRIF showed the highest homology to that of M. dolomieu. Ms_TLR3 and Ms_TRIF showed similar expression patterns in various tissues, with the highest expression level in the head kidney. After stimulation of Flavobacterium columnare, the mRNA expressions of Ms_TLR3 and Ms_TRIF were significantly up-regulated at 1 dpi in the gill, spleen and head kidney, and at 6 hpi in the trunk kidney. Furthermore, morphological changes in the gills of largemouth bass challenged with F. columnare suggested that F. columnare infection can destroy the gill filament. Taken together, Ms_TLR3 and Ms_TRIF are indeed involved in F. columnare infection and the subsequent immune response in largemouth bass. Moreover, Ms_TLR3 and Ms_TRIF might respectively play their potential roles in mucosal (mainly in the gill) and systemic (mainly in the head kidney) immune response to bacterial infection.

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