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Development and characterization of four moderate multiplex microsatellite panels in crucian carp (Carassius auratus)

文献类型: 外文期刊

作者: Cheng, Lei 1 ; Zhang, Yan 2 ; Lu, Cui-Yun 1 ; Li, Chao 1 ; Sun, Xiao-Wen 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China

2.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China

关键词: Carassius auratus;Microsatellite;Multiplex PCR;Invasion

期刊名称:CONSERVATION GENETICS RESOURCES ( 影响因子:0.973; 五年影响因子:0.946 )

ISSN: 1877-7252

年卷期: 2013 年 5 卷 3 期

页码:

收录情况: SCI

摘要: Sixteen microsatellite loci of Carassius auratus were successfully assigned into four multiplex panels in this study. These multiplex panels were characterized in 30 diploid C. auratus individuals from Songhua River. All loci were found to be polymorphic with the polymorphic information content ranging from 0.409 to 0.905. The number of alleles per locus varied from 3 to 16 and the observed heterozygosity (H (O) ) ranged from 0.269 to 0.897. Three of these loci significantly deviated from Hardy-Weinberg expectations and no pairs of loci showed significant linkage disequilibrium. These multiplex microsatellite panels will be used to determine population structure of C. auratus, and detect hybridization between closely related carps.

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