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Isolation and characterization of twenty microsatellite markers for the endangered fish Alburnus chalcoides

文献类型: 外文期刊

作者: Geng Longwu 1 ; Jiang Haifeng 1 ; Tong Guangxiang 1 ; Lu Cuiyun 1 ; Li Chao 1 ; Xu Wei 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Natl Local Joint Engn Lab Freshwater Fish Breedin, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China

关键词: Alburnus chalcoides;Microsatellite marker;Aral Sea

期刊名称:CONSERVATION GENETICS RESOURCES ( 影响因子:0.973; 五年影响因子:0.946 )

ISSN: 1877-7252

年卷期: 2013 年 5 卷 3 期

页码:

收录情况: SCI

摘要: Twenty polymorphic microsatellite loci were isolated from an enriched genomic library of Alburnus chalcoides. The polymorphism of these loci was tested on a population of 15 individuals from the Aral Sea, Uzbekistan. The number of alleles per locus ranged from 2 to 3. The observed and expected heterozygosity ranged from 0.2077-0.6720 to 0.1662-0.6837, respectively. One locus (CCA147) deviated from the Hardy-Weinberg expectations (P < 0.05) after Bonferroni correction. The polymorphic information content (PIC) for allelic diversity ranged from 0.12 to 0.8067 with 9 of these 20 loci revealing moderately high information content (PIC > 0.50). These loci will provide insights into species genetic diversity, population structure, and conservation strategy design.

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