文献类型： 外文期刊
作者： Gao, Ting 1 ; Tan, Yiqing 1 ; Wang, Yanjun 1 ; Yuan, Fangyan 1 ; Liu, Zewen 1 ; Yang, Keli 1 ; Liu, Wei 1 ; Guo, Rui 1 ; Li, Chang 1 ; Tian, Yongxiang 1 ; Zhou, Danna 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Prov Key Lab Anim Pathogen Microbiol, Key Lab Prevent & Control Agents Anim Bacteriosis, Wuhan 430064, Peoples R China
2.Huazhong Univ, Coll Vet Med, Cooperat Innovat Ctr Sustainable Pig Prod, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
关键词： Streptococcus suis; theaflavin; suilysin; molecular docking; hemolytic activity
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 24 卷 8 期
页码：
收录情况： SCI
摘要： Streptococcus suis (S. suis) is one of the most important zoonotic pathogens that threaten the lives of pigs and humans. Even worse, the increasingly severe antimicrobial resistance in S. suis is becoming a global issue. Therefore, there is an urgent need to discover novel antibacterial alternatives for the treatment of S. suis infection. In this study, we investigated theaflavin (TF1), a benzoaphenone compound extracted from black tea, as a potential phytochemical compound against S. suis. TF1 at MIC showed significant inhibitory effects on S. suis growth, hemolytic activity, and biofilm formation, and caused damage to S. suis cells in vitro. TF1 had no cytotoxicity and decreased adherent activity of S. suis to the epithelial cell Nptr. Furthermore, TF1 not only improved the survival rate of S. suis-infected mice but also reduced the bacterial load and the production of IL-6 and TNF-alpha. A hemolysis test revealed the direct interaction between TF1 and Sly, while molecular docking showed TF1 had a good binding activity with the Glu198, Lys190, Asp111, and Ser374 of Sly. Moreover, virulence-related genes were downregulated in the TF1-treated group. Collectively, our findings suggested that TF1 can be used as a potential inhibitor for treating S. suis infection in view of its antibacterial and antihemolytic activity.
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