Preparation of gutta-percha through fermentative degradation of Eucommia ulmoides Oliver leaves by recombinant yeast expressing cellulases from Trichoderma reesei
文献类型： 外文期刊
作者： Xie, Yufeng 1 ; Qin, Lijun 1 ; Zhao, Degang 1 ;
作者机构： 1.Guizhou Univ, Inst Agrobioengn, Minist Educ, Key Lab Plant Resources Conservat & Germplasm Inno, Guiyang 550025, Peoples R China
2.Guizhou Univ, Coll Life Sci, Guiyang 550025, Peoples R China
3.Guizhou Acad Agr Sci, Guizhou Plant Conservat Technol Ctr, Guiyang 550006, Peoples R China
关键词： Eucommia ulmoides; Saccharomyces cerevisiae; Cellulase gene; Fermentation; Gutta-percha
期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.9； 五年影响因子：6.0 ）
ISSN： 0926-6690
年卷期： 2023 年 192 卷
页码：
收录情况： SCI
摘要： Efficient extraction of gutta-percha from Eucommia ulmoides is important to enable its large-scale utilization in the industry. Enzymatic action of organisms offers a mild, effective and cheap technology for extracting natural products by destroying the outer wall of cells, and releasing the intracellular metabolites. In this study, Saccharomyces cerevisiae was engineered to express the recombinant cellulase genes, Cel5A/Cel6A, from Trichoderma reesei, which was then used to extract gutta-percha from the leaves of E. ulmoides. The leaves were pretreated with NaOH, ultrasound and cellulase. The results showed that when the leaves of E. ulmoides were pretreated with ultrasound (20 kHz, 30 min), with a fermentation temperature of 30 & DEG;C at pH 7, the extraction rate of gutta-percha was the highest (2.35 %). Further determination of the molecular characteristics and the thermal stability of gutta-percha using the thermogravimetric analyzer and size-exclusion chromatography revealed no significant difference in thermogravimetrics between the treatment groups. After analyzing the DCS (Differential scanning calorimetry) and TG (Thermogravimetry) curves, it was found that the gutta-percha was of & alpha;-crystal type and had a good thermal stability at 300 & DEG;C. It had the highest average molecular weight of 29.283 x 104 in the NaOH pretreatment group. This study provides a new strategy for the extraction of gutta-percha from E. ulmoides Oliver leaves.
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