BrDMC1, a Recombinase Gene, Is Involved in Seed Germination in Brassica rapa under Salt Stress
文献类型： 外文期刊
作者： Wang, Xulin 1 ; Xie, Zhengqing 1 ; Tian, Zhaoran 1 ; Wang, Shuaipeng 1 ; Shi, Gongyao 1 ; Chen, Weiwei 1 ; Cao, Gangqiang 1 ; Tian, Baoming 1 ; Wei, Xiaochun 2 ; Zhang, Luyue 1 ; Wei, Fang 1 ;
作者机构： 1.Zhengzhou Univ, Sch Agr Sci, Henan Int Joint Lab Crop Gene Resources & Improvem, Zhengzhou 450001, Peoples R China
2.Zhengzhou Univ, Henan Acad Agr Sci, Inst Hort, Grad T&R Base, Zhengzhou 450002, Peoples R China
关键词： Brassica rapa; BrDMC1; RecA; salt stress; reactive oxygen species; seed germination
期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）
ISSN：
年卷期： 2023 年 13 卷 2 期
页码：
收录情况： SCI
摘要： Recombinases are in part responsible for homologous recombination and genome integrity during DNA repair. DMC1 has a typical RecA domain, and belongs to the recombinase superfamily. The reactive oxygen species (ROS) as a potent DNA damage agent is produced during seed germination under stress conditions. DNA repair should be initiated immediately to allow for subsequent seedling development. In this study, we attempted to characterize the underlying mechanism of BrDMC1 responsiveness to salinity stress using the RNA interference approach in Brassica rapa (B. rapa). Bioinformatics and expression pattern analysis revealed that BrDMC1 only retained BrDMC1.A01 after the whole genome triplication (WGT) event and was primarily transcribed in flowers and seeds. BrDMC1 had high activity in the promoter region during germination, according to histochemical GUS staining. The data showed that salt treatment reduced the germination rate, weakened seed vigor and decreased antioxidant enzyme activity, but increased oxidative damage in BrDMC1-RNAi seeds. Furthermore, the expression of stress-responsive genes and damage repair genes was significantly different in transgenic lines exposed to salt stress. Therefore, BrDMC1 may respond to salt stress by controlling seed germination and the expression of stress-related and damage repair genes in B. rapa.
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