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Cytological and transcriptomic analyses provide insights into the pollen fertility of synthetic allodiploid Brassica juncea hybrids

文献类型： 外文期刊

作者： Wang, Boyang 1 ; Liang, Niannian 1 ; Shen, Xiaohan 1 ; Xie, Zhengqing 1 ; Zhang, Luyue 1 ; Tian, Baoming 1 ; Yuan, Yuxiang 2 ; Guo, Jialin 1 ; Zhang, Xiaowei 2 ; Wei, Fang 1 ; Wei, Xiaochun 2 ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, Henan Int Joint Lab Crop Gene Resource & Improveme, Zhengzhou 450001, Peoples R China

2.Zhengzhou Univ, Inst Hort, Henan Acad Agr Sci, Grad T&R Base, Zhengzhou 450002, Peoples R China

关键词： Interspecific hybridization; Meiosis; Mitosis; Pollen; Transcriptome; Brassica juncea

期刊名称：PLANT CELL REPORTS （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0721-7714

年卷期： 2024 年 43 卷 1 期

页码：

收录情况： SCI

摘要： Interspecific hybridization is considered to be a major pathway for species formation and evolution in angiosperms, but the occurrence of pollen abortion in the hybrids is common, prompting us to recheck male gamete development in allodiploid hybrids after the initial combination of different genomes. Here, we investigated the several key meiotic and mitotic events during pollen development using the newly synthesised allodiploid B. juncea hybrids (AB, 2n = 2x = 18) as a model system. Our results demonstrated the partial synapsis and pairing of non-homologous chromosomes concurrent with chaotic spindle assembly, affected chromosome assortment and distribution during meiosis, which finally caused difference in genetic constitution amongst the final tetrads. The mitotic cell cycle arrest during microspore development resulted in the production of anucleate pollen cells. Transcription analysis showed that sets of key genes regulating cyclin (CYCA1;2 and CYCA2;3), DNA damage repair (DMC1, NBS1 and MMD1), and ubiquitin-proteasome pathway (SINAT4 and UBC) were largely downregulated at the early pollen meiosis stages, and those genes involved in sperm cell differentiation (DUO1, PIRL1, PIRL9 and LBD27) and pollen wall synthesis (PME48, VGDH11 and COBL10) were mostly repressed at the late pollen mitosis stages in the synthetic allodiploid B. juncea hybrids (AB). In conclusion, this study elucidated the related mechanisms affecting pollen fertility during male gametophyte development at the cytological and transcriptomic levels in the synthetic allodiploid B. juncea hybrids.

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