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The critical roles of the Zn2 Cys6 transcription factor Fp487 in the development and virulence of Fusarium pseudograminearum : A potential target for Fusarium crown rot control

文献类型： 外文期刊

作者： Yang, Xiaoyue 1 ; Cao, Shulin 1 ; Sun, Haiyan 1 ; Deng, Yuanyu 1 ; Zhang, Xin 1 ; Li, Yan 2 ; Ma, Dongfang 2 ; Chen, Huaigu 1 ; Li, Wei 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

2.Yangtze Univ, Coll Agr, Key Lab Sustainable Crop Prod Middle Reaches Yangt, Jingzhou 434025, Hubei, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Yangzhou 225009, Jiangsu, Peoples R China

关键词： Zn 2 Cys 6 transcription factor; FCR; Wheat; Fusarium pseudograminearum; Virulence; Spray-induced gene silencing

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.1； 五年影响因子：6.5 ）

ISSN： 0944-5013

年卷期： 2024 年 285 卷

页码：

收录情况： SCI

摘要： Fusarium crown rot (FCR) caused by Fusarium pseudograminearum poses a significant threat to wheat production in the Huang-Huai-Hai region of China. However, the pathogenic mechanism of F. pseudograminearum is still poorly understood. Zn 2 Cys 6 transcription factors, which are exclusive to fungi, play pivotal roles in regulating fungal development, drug resistance, pathogenicity, and secondary metabolism. In this study, we present the functional characterization of a Zn 2 Cys 6 transcription factor F. pseudograminearum , designated Fp487 . In F. pseudograminearum , Fp487 is shown to be required for mycelial growth through gene knockout and phenotypic analyses. Compared with wild -type CF14047, the Delta Fp487 mutant displayed a slight reduction in growth rate but a significant decrease in conidiogenesis, pathogenicity and 3-acetyl-deoxynivalenol (3AcDON) production. Moreover, the mutant exhibited heightened sensitivity to oxidative and cytomembrane stress. Furthermore, we synthesized dsRNA from the Fp487 gene in vitro , resulting in a reduction in the growth rate of F. pseudograminearum and its virulence on barley leaves through spray-induced gene silencing (SIGS). Notably, this study makes the first instance of inducing the expression of abundant dsRNA from F. pseudograminearum by engineering the Escherichia coli strain HT115 (DE3) and utilizing the SIGS technique to evaluate the virulence effect of dsRNA on F. pseudograminearum . In conclusion, our findings revealed the crucial role of Fp487 in regulating pathogenicity, stress responses, DON production, and conidiogenesis in F. pseudograminearum . Furthermore, Fp487 is a potential RNAi-based target for FCR control.

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