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Knock-down of amh transcription by antisense RNA reduces FSH and increases follicular atresia in female Oreochromis niloticus

文献类型： 外文期刊

作者： Qiang, Jun 1 ; Cao, Zhe-Ming 1 ; Zhu, Hao-Jun 1 ; Tao, Yi-Fan 1 ; He, Jie 1 ; Xu, Pao 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resources, Wuxi, Jiangsu, Peoples R China

关键词： Antisense RNA; Oreochromis niloticus; amh transcription inhibition; AMH signaling; Developmental degeneration

期刊名称：GENE （ 影响因子：3.913； 五年影响因子：3.48 ）

ISSN： 0378-1119

年卷期： 2022 年 842 卷

页码：

收录情况： SCI

摘要： Anti-Mullerian hormone (Amh) plays an important role in regulating gonad development in teleosts. However, little is known about the effects of Amh on follicle development. In this study, we transfected the vector con-taining antisense RNA fragments of the amh gene to produce Nile tilapia, Oreochromis niloticus, with knocked -down Amh function in vivo. The results confirmed that the antisense RNA effectively inhibited amh transcrip-tion and Amh protein expression in female tilapia ovarian tissue. At 180 days of age, compared with control fish, female tilapia with knocked-down Amh function showed significantly increased growth and significantly decreased ovary weight and gonadosomatic index (P < 0.05). Female fish in the control group had ruddy-colored external genitalia, eggs extruded from the abdomen when gently squeezed, and most oocytes were develop-mental stage V. In contrast, the external genitalia of female fish with knocked-down Amh function did not have the ruddy color, no eggs extruded from the abdomen when squeezed, most oocytes were at developmental stages II and III, and considerable follicular atresia was apparent. At 180 days of age, the transcript levels of amhrII, cyp19a1a, foxl2 and sox9b in ovarian tissue, and the titers of luteinizing hormone, follicle stimulating hormone, and estradiol in the serum, were significantly lower in fish with knocked-down Amh function than in control fish (P < 0.05). We concluded that decreased serum hormone levels and an abnormal AMH signal delayed devel-opment and caused follicular degeneration in Nile tilapia with knocked-down Amh function. These findings show that antisense RNA is a feasible approach for gene silencing in fish, and represents an accurate and effective strategy to study gene function.

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