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The viable but non-culturable (VBNC) status of Shewanella putrefaciens (S. putrefaciens) with thermosonication (TS) treatment

文献类型： 外文期刊

作者： Jiang, Ziwei 1 ; Wang, Yi 1 ; Bai, Shunjie 1 ; Bai, Chan 1 ; Tu, Ziyi 4 ; Li, Hailan 1 ; Guo, Peng 1 ; Liao, Tao 1 ; Qiu, Liang 1 ;

作者机构： 1.Hubei Acad Agr Sci, Key Lab Cold Chain Logist Technol Agroprod, Minist Agr & Rural Affairs, Inst Agroprod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China

2.Hubei Engn Res Ctr Agroprod Irradiat, Agroprod Proc Res Subctr Hubei Innovat Ctr Agr Sci, Wuhan 430064, Peoples R China

3.Wuhan Inst Technol, Sch Chem & Environm Engn, Wuhan 4300731, Peoples R China

4.HuBei Crawfish Ind Tech Ltd, Qianjiang 433100, Peoples R China

关键词： Shewanella putrefaciens; Thermosonication treatment; VBNC status; Protein regulation

期刊名称：ULTRASONICS SONOCHEMISTRY （ 影响因子：8.7； 五年影响因子：7.9 ）

ISSN： 1350-4177

年卷期： 2024 年 109 卷

页码：

收录情况： SCI

摘要： Although thermosonication (TS) treatment has been widely used in food sterilization, the viable but non-culturable (VBNC) of bacteria with TS treatment has still concerned potential food safety and public health. The molecular mechanism of VBNC status of bacteria with TS treatment is not clearly known. Therefore, in this study, we used Shewanella putrefaciens, which was a common putrefactive bacteria in aquatic products, to study the VBNC state of bacteria with TS treatment. Firstly, our results revealed that S. putrefaciens still could enter the VBNC state after TS treatments: 50 kHz, 300 W, 30 min ultrasonic treatment and 70 degrees C heating; Subsequently, we found the VBNC state of S. putrefaciens can resist the damage of TS treatment, such as cell wall break, DNA degradation, etc; Finally, four-dimensional data-independent acquisition-based proteomics showed that under VBNC state, S. putrefaciens upregulated functional proteins to resist TS treatment, such as: ribosomal proteins to accelerate the synthesis of stress proteins to counteract TS treatments, ornithine decarboxylase SpeF and MraY to repair TS treatment-induced damage, etc. Meanwhile, S. putrefaciens downregulates metabolic and transport functional proteins such as dehydrogenase to reduce the metabolism. Importantly, among those proteins, the ribosomal transcriptional regulatory protein family, such as rpsB, etc, may be the key proteins for S. putrefaciens entering VBNC state. This finding can provide some new strategies for preventing VBNC status of bacteria with TS treatment, such as: inhibition of key proteins, etc.

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