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Effects of autophagy inhibition by 3-methyladenine on encystation, morphology, and metabolites of Cryptocaryon irritans

文献类型： 外文期刊

作者： Bushra, Ivon F. 1 ; Maha, Ivon F. 1 ; Yu, Youbin 2 ; Jiang, Linhua 3 ; Xie, Xiao 1 ; Zhou, Suming 1 ; Yin, Fei 1 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Appl Marine Biotechnol,Minist Educ, 818 Fenghua Rd, Ningbo 315211, Peoples R China

2.Chinese Acad Fishery Sci, Fishery Machinery & Instrument Res Inst, Key Lab Ocean Fishing Vessel & Equipment, Minist Agr & Rural Affairs, 63 Chifeng Rd, Shanghai 200092, Peoples R China

3.Huzhou Univ, Sch Informat Engn, Huzhou 313000, Peoples R China

关键词： Cryptocaryon irritans; Encystation; Autophagy inhibition; Ultrastructure; Metabolomics; 3-Methyladenine

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.383； 五年影响因子：2.348 ）

ISSN： 0932-0113

年卷期：

页码：

收录情况： SCI

摘要： Encystment is crucial for defense and reproduction in Cryptocaryon irritans. Therefore, understanding the encystment-related events in the protomont stage can help prevent and control C. irritans. Autophagy promotes protozoan parasite encystation. However, 3MA can inhibit autophagy. In this study, the effects of autophagy inhibition on encystation, survival rate, ultrastructural features, and metabolomic profiles of C. irritans, were evaluated using protomonts treated with 3MA (20 mM). The treatment with 3MA for about 4 h significantly lowered survival and encystation rates of protomonts to about 86.44% and 76.08%, respectively. Microstructural observations showed that the 3MA-treated protomonts showed deformed cell membranes and the cytoplasmic content spill. Furthermore, observation of the ultrastructure of 3MA-treated protomonts showed the destruction of organelles (Golgi bodies and mucocyst) and a lack of autophagosomes. However, no abnormality was observed in the control experiments. Furthermore, the metabolic analysis revealed suppression of metabolites, such as lipids, amino acids, and carbohydrates. These results demonstrate that 3MA can inhibit autophagy in C. irritans, thus hindering encystation, suppressing the metabolism of metabolites, and altering morphological ultrastructure in these parasites.

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