Genetic diversity of Listeria monocytogenes isolates from farm products in Shanghai, China
文献类型： 外文期刊
作者： Suo, Yujuan 1 ; Qu, Yang 1 ; Bai, Yalong 1 ; Zhou, Xiujuan 2 ; Lin, Ting 1 ; Wang, Jing 1 ; Chen, Wanyi 3 ; Zhou, Changyan 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Agroprod, Shanghai, Peoples R China
2.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, Shanghai, Peoples R China
3.Changshu Inst Technol, Sch Biol & Food Engn, Changshu, Jiangsu, Peoples R China
关键词： Listeria monocytogenes; serotype; multilocus sequence typing (MLST); pulsed field gel electrophoresis (PFGE); whole-genome single nucleotide polymorphism (wgSNP)
期刊名称：FOOD QUALITY AND SAFETY （ 影响因子：3.949； 五年影响因子：6.469 ）
ISSN： 2399-1399
年卷期： 2022 年 6 卷
页码：
收录情况： SCI
摘要： Farm products are considered important vehicles for the transmission of Listeria monocytogenes (L. monocytogenes). The typing of L. monocytogenes from farm products contributes to the surveillance and source tracing of the pathogen. In this study, 77 L. monocytogenes strains from seven farm product categories in Shanghai were analyzed by serological typing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome nucleotide polymorphism (wgSNP). The results showed that the 77 isolates were classified into four serovars (1/2a, 1/2c, 1/2b, and 4b), and of these, 1/2a (n=47) was the most prevalent. Seventeen sequence types (STs) were generated by MLST with two novel STs (ST1402 and ST1403), and 20 of 77 L. monocytogenes isolates had high genetic identity with previously documented outbreak isolates according to the minimum spanning tree from the MLST results. Moreover, 34 PFGE patterns (PF1-PF34) were differentiated, and based on a similarity value higher than 80% by the unweighted pair group method dendrogram, the discriminatory index was relatively low (equal to 0.775). Furthermore, 14 isolates were chosen and further analyzed by wgSNP based on the previous typing results, which demonstrated that wgSNP and MLST yielded mostly consistent typing results but higher resolution than PFGE. In conclusion, 77 L. monocytogenes isolates from farm products collected in nine districts in Shanghai were highly genetically diverse, and 20 of these isolates had high relatedness with previously documented outbreak strains worldwide. The results indicate a possible cross-contamination risk of L. monocytogenes and a potential public health concern resulting from farm products during the supply chain in Shanghai, China.
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