Application of engineered heme enzymes based on myoglobin with high peroxidase activity for efficient degradation of various emerging pollutants
文献类型： 外文期刊
作者： Wu, Guang-Rong 1 ; Xu, Jia-Kun 2 ; Sun, Li-Juan 3 ; Li, Ying-Fu 1 ; Gao, Shu-Qin 3 ; Lin, Ying-Wu 1 ;
作者机构： 1.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Drugs & Byprod,Pilot Natl Lab Marine Sc, Qingdao 266071, Peoples R China
3.Univ South China, Hengyang Med Sch, Hengyang 421001, Peoples R China
关键词： Emerging pollutants; Heme enzyme; Myoglobin; Biodegradation
期刊名称：JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING （ 影响因子：7.7； 五年影响因子：7.3 ）
ISSN： 2213-2929
年卷期： 2023 年 11 卷 6 期
页码：
收录情况： SCI
摘要： Emerging pollutants in the aquatic environment from pharmaceutical and personal care products (PPCPs) attract much attention due to their environmental risk and toxicological properties, posing a threat to human health and safety. In this study, we fulfilled the direct and efficient degradation of PPCPs (i.e. diclofenac sodium, 2-mercap-tobenzothiazole, paracetamol, and furosemide) using engineered H2O2-dependent heme enzymes based on myoglobin (Mb) with high peroxidase activity. Especially, the triple mutant F43Y/P88W/F138W Mb could efficiently degrade diclofenac sodium, 2-mercaptobenzothiazole, paracetamol, and furosemide with a degrada-tion rate of-98.5%,-99%,-98%, and-91%, respectively. These activities are much higher than other re-ported native enzymes, such as laccase and horseradial peroxidase. As further analyzed by ESI-MS, we identified multiple degradation products of those four contaminants and thus proposed possible degradation mechanisms. This study indicates that enzyme-based degradation of emerging pollutants is a promising environmental remediation approach.
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