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Signaling pathways activated by sea bass gonadotropin-inhibitory hormone peptides in COS-7 cells transfected with their cognate receptor

文献类型： 外文期刊

作者： Wang, Bin 1 ; Paullada-Salmeron, Jose A. 1 ; Verges-Castillo, Alba 1 ; Gomez, Ana 7 ; Munoz-Cueto, Jose A. 1 ;

作者机构： 1.Univ Cadiz, Fac Marine & Environm Sci, Dept Biol, Cadiz, Spain

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao, Peoples R China

3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries & Food Prod Proc, Qingdao, Peoples R China

4.Marine Campus Int Excellence CEIMAR, Marine Res Inst INMAR, Cadiz, Spain

5.Agrifood Campus Int Excellence CeiA3, Cadiz, Spain

6.European Univ Seas SEA EU, Cadiz, Spain

7.CSIC, Inst Aquaculture Torre Sal, Castellon de La Plana, Spain

关键词： GnIH; GnIH receptor; kisspeptin; kisspeptin receptor; signaling pathway

期刊名称：FRONTIERS IN ENDOCRINOLOGY （ 影响因子：6.055； 五年影响因子：6.335 ）

ISSN： 1664-2392

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Results of previous studies provided evidence for the existence of a functional gonadotropin-inhibitory hormone (GnIH) system in the European sea bass, Dicentrarchus labrax, which exerted an inhibitory action on the brain-pituitary-gonadal axis of this species. Herein, we further elucidated the intracellular signaling pathways mediating in sea bass GnIH actions and the potential interactions with sea bass kisspeptin (Kiss) signaling. Although GnIH1 and GnIH2 had no effect on basal CRE-luc activity, they significantly decreased forskolin-elicited CRE-luc activity in COS-7 cells transfected with their cognate receptor GnIHR. Moreover, an evident increase in SRE-luc activity was noticed when COS-7 cells expressing GnIHR were challenged with both GnIH peptides, and this stimulatory action was significantly reduced by two inhibitors of the PKC pathway. Notably, GnIH2 antagonized Kiss2-evoked CRE-luc activity in COS-7 cells expressing GnIHR and Kiss2 receptor (Kiss2R). However, GnIH peptides did not alter NFAT-RE-luc activity and ERK phosphorylation levels. These data indicate that sea bass GnIHR signals can be transduced through the PKA and PKC pathways, and GnIH can interfere with kisspeptin actions by reducing its signaling. Our results provide additional evidence for the understanding of signaling pathways activated by GnIH peptides in teleosts, and represent a starting point for the study of interactions with multiple neuroendocrine factors on cell signaling.

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