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Role of AcndtA in cleistothecium formation, osmotic stress response, pigmentation and carbon metabolism of Aspergillus cristatus

文献类型： 外文期刊

作者： Wang, Yaping 1 ; Tan, Yumei 2 ; Wang, Yuchen 2 ; Ge, Yongyi 4 ; Liu, Yongxiang 2 ; Liu, Hui 2 ; Shao, Lei 1 ; Liu, Yimei; 1 ;

作者机构： 1.Guizhou Univ, Coll Agr, Guiyang 550025, Guizhou, Peoples R China

2.Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Guizhou, Peoples R China

3.Guizhou Acad Agr Sci, Inst Biotechnol, Guiyang 550006, Guizhou, Peoples R China

4.Guizhou Univ, Coll Life & Sci, Guiyang 550025, Guizhou, Peoples R China

5.Chibi Chebu Senior High Sch, Chibi 437300, Huibei, Peoples R China

6.Guizhou Univ Tradit Chinese Med, Guiyang 550025, Guizhou, Peoples R China

7.Zhongkai Univ Agr & Engn, Innovat Inst Plant Hlth, Guangzhou 510225, Guangdong, Peoples R China

关键词： Sexual development; Asexual sporulation; NDT80; RNA-Seq

期刊名称：FUNGAL BIOLOGY （ 影响因子：3.099； 五年影响因子：3.364 ）

ISSN： 1878-6146

年卷期： 2021 年 125 卷 10 期

页码：

收录情况： SCI

摘要： As the dominant fungus during the fermentation of Fuzhuan brick tea, Aspergillus cristatus is easily induced to undergo a sexual cycle under low-salt stress. However, the underlying regulatory mechanism of sexual reproduction is unclear. Here, we report a P53-like transcription factor AcndtA, which encodes an NDT80 DNA binding protein and regulates fungal reproduction, pigmentation and the stress response. Both insertion and deletion mutants of AcndtA exhibited a complete blockade of cleistothecium formation, and overexpressing AcndtA strains (OE: AcndtA) exhibited significantly reduced cleistothecium production, indicating that AcndtA plays a vital role in sexual development. Osmotic stress tests showed that overexpression of AcndtA had a negative impact on growth and conidia production. Additionally, AcndtA insertion, deletion and overexpression mutants exhibited reduced pigment formation. All the above developmental defects were reversed by the re-introduction of the AcndtA gene in DAcndtA. Moreover, the growth of AcndtA mutants in carbon-limited medium was better than that of the WT and OE: AcndtA strains, indicating that AcndtA is involved in carbon metabolism. Transcriptional profiling data showed that AcndtA regulated the expression of several genes related to development, osmotic stress and carbon metabolism. (c) 2021 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

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