文献类型： 外文期刊

作者： Wu, Youzhen ¹ ; Tan, Yumei ² ; Peng, Qiuju ¹ ; Xiao, Yang ³ ; Xie, Jiaofu ⁴ ; Li, Zhu ¹ ; Ding, Haixia ⁵ ; Pan, Hang ¹ ; Wei, Longfeng ¹ ;

作者机构： 1.Guizhou Univ, Inst Agrobioengn, Coll Life Sci, Key Lab Plant Resource Conservat & Germplasm Innov, Guiyang, Guizhou, Peoples R China

2.Guizhou Acad Agr Sci, Inst Biotechnol, Guizhou Key Lab Agr Biotechnol, Guiyang, Guizhou, Peoples R China

3.Inst Supervis & Inspect Prod Qual Guizhou Prov, Guiyang, Guizhou, Peoples R China

4.Guiyang 1 High Sch, Guiyang, Guizhou, Peoples R China

5.Guizhou Univ, Coll Agr, Dept Plant Pathol, Guiyang, Guizhou, Peoples R China

关键词： Endophytic bacterium; Camellia sinensis; Colletotrichum gloeosporioides; Bacillus altitudinis; Biocontrol efficacy

期刊名称：PEERJ （ 影响因子：2.7； 五年影响因子：3.1 ）

ISSN： 2167-8359

年卷期： 2024 年 12 卷

页码：

收录情况： SCI

摘要： Background: As one of the main pathogens causing tea anthracnose disease, Colletotrichum gloeosporioides has brought immeasurable impact on the sustainable development of agriculture. Given the adverse effects of chemical pesticides to the environment and human health, biological control has been a focus of the research on this pathogen. Bacillus altitudinis GS-16, which was isolated from healthy tea leaves, had exhibited strong antagonistic activity against tea anthracnose disease.Methods: The antifungal mechanism of the endophytic bacterium GS-16 against C. gloeosporioides 1-F was determined by dual-culture assays, pot experiments, cell membrane permeability, cellular contents, cell metabolism, and the activities of the key defense enzymes.Results: We investigated the possible mechanism of strain GS-16 inhibiting 1-F. In vitro, the dual-culture assays revealed that strain GS-16 had significant antagonistic activity (92.03%) against 1-F and broad-spectrum antifungal activity in all tested plant pathogens. In pot experiments, the disease index decreased to 6.12 after treatment with GS-16, indicating that strain GS-16 had a good biocontrol effect against tea anthracnose disease (89.06%). When the PE extract of GS-16 treated mycelial of 1-F, the mycelial appeared deformities, distortions, and swelling by SEM observations. Besides that, compared with the negative control, the contents of nucleic acids, protein, and total soluble sugar of GS-16 group were increased significantly, indicating that the PE extract of GS-16 could cause damage to integrity of 1-F. We also found that GS-16 obviously destroyed cellular metabolism and the normal synthesis of cellular contents. Additionally, treatment with GS-16 induced plant resistance by increasing the activities of the key defense enzymes PPO, SOD, CAT, PAL, and POD.Conclusions: We concluded that GS-16 could damage cell permeability and integrity, destroy the normal synthesis of cellular contents, and induce plant resistance, which contributed to its antagonistic activity. These findings indicated that strain GS-16 could be used as an efficient microorganism for tea anthracnose disease caused by C. gloeosporioides.