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Perception of the Biocontrol Potential and Palmitic Acid Biosynthesis Pathway of Bacillus subtilis H2 through Merging Genome Mining with Chemical Analysis

文献类型： 外文期刊

作者： Pan, Hang 1 ; Wei, Longfeng 1 ; Zhao, Hao 1 ; Xiao, Yang 2 ; Li, Zhu 1 ; Ding, Haixia 4 ;

作者机构： 1.Guizhou Univ, Inst Agrobioengn, Coll Life Sci, Key Lab Plant Resource Conservat & Germplasm Innov, Guiyang 550025, Guizhou, Peoples R China

2.Inst Supervis & Inspect Prod Qual Guizhou Prov, Guiyang 550004, Peoples R China

3.Guizhou Acad Agr Sci, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China

4.Guizhou Univ, Coll Agr, Dept Plant Pathol, Guiyang 550025, Peoples R China

关键词： Bacillus subtilis; antagonistic activity; comparativegenomic analysis; palmitic acid (PA); Magnaportheoryzae; field efficacy

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Bacillus has been widely studied for its potential to protect plants from pathogens. Here, we report the whole genome sequence of Bacillus subtilis H2, which was isolated from the tea garden soil of Guiyang Forest Park. Strain H2 showed a broad spectrum of antagonistic activities against many plant fungal pathogens and bacteria pathogens, including the rice blast fungus Magnaporthe oryzae, and showed a good field control effect against rice blast. The complete genome of B. subtilis H2 contained a 4,160,635-bp circular chromosome, with an average G + C content of 43.78%. Through the genome mining of strain H2, we identified 7 known antimicrobial compound biosynthetic gene clusters (BGCs) including sporulation killing factor, surfactin, bacillaene, fengycin, bacillibactin, subtilosin A, and bacilysin. Palmitic acid (PA), a secondary metabolite, was detected and identified in the H2 strain through genome mining analysis and gas chromatography-mass spectrometry (GC-MS). Additionally, we propose, for the first time, that the type II fatty acid synthesis (FAS) pathway in Bacillus is responsible for PA biosynthesis. This finding was confirmed by studying the antimicrobial activity of PA and conducting reverse transcription-quantitative polymerase chain reaction (RT-qPCR) experiments. We also identified numerous genes associated with plant-bacteria interactions in the H2 genome, including more than 94 colonization-related genes, more than 34 antimicrobial genes, and more than 13 plant growth-promoting genes. These findings contribute to our understanding of the biocontrol mechanisms of B. subtilis H2 and have potential applications in crop disease control.

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[1]Perception of the Biocontrol Potential and Palmitic Acid Biosynthesis Pathway of Bacillus subtilis H2 through Merging Genome Mining with Chemical Analysis. Pan, Hang,Wei, Longfeng,Zhao, Hao,Li, Zhu,Li, Zhu,Ding, Haixia,Xiao, Yang. 2024

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