您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A new strain of yellow catfish carrying genome edited myostatin alleles exhibits double muscling phenotype with hyperplasia

文献类型： 外文期刊

作者： Zhang, Xincheng 1 ; Wang, Fang 1 ; Dong, Zhangji 2 ; Dong, Xiaohua 2 ; Chi, Jun 3 ; Chen, Haigang 1 ; Zhao, Qingshun 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr, 1 Xingyu Rd, Guangzhou 510380, Guangdong, Peoples R China

2.Nanjing Univ, Model Anim Res Ctr, 12 Xuefu Rd, Nanjing 210061, Jiangsu, Peoples R China

3.Nanjing YSY Biotech Co, Inst Genome Editing, 1 Amber Rd, Nanjing 211812, Jiangsu, Peoples R China

关键词： Yellow catfish; Myostatin; Genome editing; Double muscling; Hyperplasia; Hypertrophy

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2020 年 523 卷

页码：

收录情况： SCI

摘要： The yellow catfish (Pelteobagrus fulvidraco Richardson) is an important commercial freshwater aquaculture species in China. To generate a new strain of yellow catfish featured with good production traits, we knocked out mstna, an orthologue of mammalian myostatin gene (Mstn) encoding a negative regulator of skeletal muscle development and growth, using zinc finger nucleases. Similar to livestock carrying mutated Mstn, the genome-edited-msma yellow catfish grew and bred normally, and both the male and female homozygous mutants displayed double muscling phenotype with two obvious muscle mass sticking out between head and dorsal fin when they reached 1-month-old and become more obvious with growth. The body weight of mstna null yellow catfish was heavier than (1.27- to 1.37-fold of) their wild-type siblings at 80 dpf (days post fertilization) and 210 dpf, respectively. Histological analysis revealed that the mstna knockout yellow catfish had increasing muscle fiber number but decreasing muscle fiber size in the skeletal muscle. Our results demonstrated that genome editing technology is a good choice for us to breed a new strain of yellow catfish with accelerated growth and more muscle mass.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台