文献类型： 外文期刊
作者： Zhao, Yanyan 1 ; Liang, Ying 2 ; Liu, Yuan 2 ; Tu, Sicong 4 ; Zhao, Shengming 1 ; Liu, Xianjin 2 ; Tu, Kang 3 ;
作者机构： 1.Henan Inst Sci & Technol, Sch Food Sci & Technol, Xinxiang 453003, Henan, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Prod Qual Safety & Nutr Res, Minist Agr & Rural Affairs,Key Lab Control Techno, Key Lab Food Qual & Safety Jiangsu Prov,State Key, Nanjing 210014, Peoples R China
3.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China
4.Univ New South Wales, Sch Med Sci, Sydney, NSW 2052, Australia
关键词： domain antibody; enzyme-linked immunosorbent assay; phenoxybenzoic acid; pyrethroid
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING （ 影响因子：2.032； 五年影响因子：2.137 ）
ISSN： 1934-6344
年卷期： 2020 年 13 卷 2 期
页码：
收录情况： SCI
摘要： Anti-idiotypic antibodies which can mimic antigens have many potential applications in the immunoassay. This research used the monoclonal antibody of the conserved chemical region of pyrethroids, 3-phenoxybenzoic acid (PBA), and the domain antibody library to develop an environmentally-friendly immunoassay for the detection of pyrethroids. The domain antibodies (A8, B8, and C6) which bound to anti-PBA monoclonal antibody (MAb) were isolated from a naive phage display human domain antibody library. This domain antibody is cloneable, pyrethroid-free, and applicable as a competitive mimetic antigen in the immunoassay. The best immunoassay was achieved using A8, resulting in IC50 of 0.714 mu g/mL for PBA, 1.775 mu g/mL for Cypermethrin, 1.624 mu g/mL for beta-cypermethrin, 3.675 mu g/mL for Fenvalerate, and 4.895 mu g/mL for Flucythrinate. This way of selecting anti-idiotypic antibodies to detect pyrethroids could provide potential applications in developing immunoassays for identifying various chemical contaminants in food.
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