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OsPDR2 mediates the regulation on the development response and maintenance of Pi homeostasis in rice

文献类型： 外文期刊

作者： Cao, Yue 1 ; Jain, Ajay 2 ; Ai, Hao 1 ; Liu, Xiuli 1 ; Wang, Xiaowen 1 ; Hu, Zhi 1 ; Sun, Yafei 1 ; Hu, Siwen 1 ; Shen, Xing 1 ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Key Lab Plant Nutr & Fertilizat Low Middle Reache, Minist Agr, Nanjing 210095, Peoples R China

2.Amity Univ Rajasthan, Amity Inst Biotechnol, Jaipur, Rajasthan, India

3.Nanjing Agr Univ, Coll Hort, Landscape Architecture Dept, Nanjing 210095, Peoples R China

4.Shanghai Acad Agr Sci, Inst Ecoenvironm & Plant Protect, Shanghai 201403, Peoples R China

5.Sun Yat Sen Univ, Sch Environm Sci & Engn, Guangzhou 510275, Peoples R China

关键词： Rice (Oryza sativa); Pi deficiency; OsPDR2; RNAi-mediated suppression; Morphophysiological and molecular traits; Growth and development; Pi homeostasis

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 149 卷

页码：

收录情况： SCI

摘要： Inorganic orthophosphate (Pi), a major form of essential macronutrient phosphorus (P), is available in rhizosphere for acquisition and assimilation by plants. However, the limited availability of Pi in soils affects the growth and development of plants. In Arabidopsis thaliana (Arabidopsis), Phosphate Deficiency Response2 (AtPDR2), interacts genetically with Low Phosphate Root1 (AtLPR1) in the endoplasmic reticulum (ER) and plays a key role in the inhibition of primary root growth (PRG) during Pi deficiency. However, the role of OsPDR2, the homolog of AtPDR2, either in roots response to Pi deficiency and/or in growth and development has not been elucidated as yet. Therefore, qRT-PCR was employed to determine the spatiotemporal effects and the availability of Pi on the expression of OsPDR2. OsPDR2 showed variable levels of relative expression pattern in vegetative and/or reproductive tissues analyzed at different stages of growth and development (5-17 weeks). Transient expression analysis revealed its subcellular localization to the ER. Further, the reverse genetics approach was employed for determining the function of OsPDR2 by generating RNAi lines (Ri2, Ri9, and Ri18). The study revealed significant inhibitory effects of RNAi-mediated suppression of OsPDR2 on the development of root, male reproductive traits, and yield. Moreover, P-32 isotope labeling and split-root experiments under different Pi regime with RNAi lines revealed the function of OsPDR2 in regulating homeostasis of Pi.

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