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Downregulation of chitin synthase A gene by diallyl trisulfide, an active substance from garlic essential oil, inhibits oviposition and alters the morphology of adult Sitotroga cerealella

文献类型： 外文期刊

作者： Shah, Sakhawat 1 ; Hafeez, Muhammad 2 ; Wu, Meng-Ya 1 ; Zhang, Su-Su 1 ; Ilyas, Muhammad 3 ; Wu, Gang 1 ; Yang, Feng-L 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Hubei, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Peoples R China

3.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Hubei, Peoples R China

关键词： Diallyl trisulfide; Sitotroga cerealella; Chitin synthase A; Physiology and morphology; Ovipositional reduction

期刊名称：JOURNAL OF PEST SCIENCE （ 影响因子：5.918； 五年影响因子：5.955 ）

ISSN： 1612-4758

年卷期： 2020 年 93 卷 3 期

页码：

收录情况： SCI

摘要： The Angoumois grain moth, Sitotroga cerealella Olivier (Lepidoptera: Gelechiidae), is the most serious and destructive pest of stored commodities around the globe, particularly on wheat, rice and maize. Previously, we confirmed that diallyl trisulfide (DAT), an active substance from garlic essential oil, reduced oviposition of females after a fumigation at the sublethal concentration (LC20 = 0.015 mu L/L); meanwhile, through transcriptome sequencing, we found that DAT significantly downregulates the expression of chitin synthase A (CHSA) gene. To gain a broader insight, we assessed effects of DAT and dsRNA of CHSA gene of S. cerealella (dsScerCHSA) on the morphology, physiology and oviposition of adult S. cerealella, and we found that ScerCHSA was expressed in the adult stage and observed higher in the cuticle. Furthermore, results indicated that DAT fumigation significantly downregulated the mRNA expression of ScerCHSA and inhibited moth oviposition at the sublethal concentration. Downregulation of ScerCHSA resulted in chitin reduction, epidermal disruption, morphological and physiological alterations. Meanwhile, silencing of ScerCHSA resulted in the oviposition reduction of the moth; moreover, the morphological and physiological alterations were similar to the group treated with DAT. Therefore, we conclude that DAT could regulate the expression of ScerCHSA at the transcriptional level, cause histological and morphological alteration and finally inhibit the moth oviposition, which proves DAT possesses the great potential for being used in pest control as a friendly biopesticide.

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