Proteomic alteration of porcine intestinal epithelial cells after pretreatment with Lactobacillus plantarum followed by infection with enterotoxigenic Escherichia coli F4
文献类型： 外文期刊
作者： Zhu, Cui 1 ; Lv, Yantao 1 ; Yang, Jun 1 ; Bai, Yinshan 2 ; Ye, Jinling 1 ; Wang, Zhilin 1 ; Chen, Zhuang 1 ; Jiang, Zongy 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Peoples R China
2.Foshan Univ, Sch Life Sci & Engn, Foshan 528225, Peoples R China
3.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China
关键词： Proteomic analysis; IPEC-J2 cells; Lactobacillus plantarum; Enterotoxigenic Escherichia coli; Weaned piglet; Diarrhea
期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）
ISSN： 0165-2427
年卷期： 2020 年 222 卷
页码：
收录情况： SCI
摘要： Enterotoxigenic Escherichia coli (ETEC) F4 causes diarrhea in infants and weaned piglets. The technique of isobaric tags for relative and absolute quantitation (iTRAQ) was used in this study to determine the differentially expressed proteins in porcine intestinal epithelial cells (IPEC-J2) after pretreatment with Lactobacillus plantarum (LP) followed by challenge with ETEC F4. A total of 4771 proteins were identified in IPEC-J2 cells, with 90, 105, and 134 differentially expressed proteins in cells exposed to ETEC, LP, and LP + ETEC, respectively. The COG analysis divided the identified proteins into 20 categories. The GO and KEGG annotation indicated that most of the differentially expressed proteins were enriched in various biological metabolism including cell cycle control, cell division and differentiation. Additionally, western blotting analyses confirmed the reduced abundance of selected proteins of the mTOR and MAPK signal pathways affected by ETEC F4. Moreover, LP pretreatment increased JNK activation in IPEC-J2 cells infected with ETEC F4. These results may provide further insights into the mechanisms involved in the interaction between ETEC F4 and intestinal epithelial cells, and broaden the understanding of the protective effects of LP in alleviating ETEC-provoked diarrhea of piglets.
- 相关文献
作者其他论文 更多>>
-
Effect of mixed meal replacement of soybean meal on growth performance, nutrient apparent digestibility, and gut microbiota of finishing pigs
作者：He, Zhentao;Liu, Shuai;Wen, Xiaolu;Cao, Shuting;Zhan, Xianliang;Hou, Lei;Li, Yaojie;Chen, Shaozhen;Zheng, Huayu;Deng, Dongyan;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wang, Li
关键词：rapeseed meal; cotton meal; sunflower meal; finishing pigs; short-chain fatty acid
-
Integrative Analysis of the Microbiome and Metabolome of Broiler Intestine: Insights into the Mechanisms of Probiotic Action as an Antibiotic Substitute
作者：Li, Yang;Chen, Zhuang;Liu, Shi;Liu, Jing;Huang, Wen-Jie;Deng, Jun-Jin;Wang, Zhi-Lin;Zhang, Jian-Ling;Li, Jia-Zhou;Deng, Jun-Jin;Li, Jia-Zhou;Ruan, Dong;Chen, Jia-Qi
关键词：Broiler breeding; Antibiotic substitute; Growth performance; Microbiome; Metabolome
-
Haplotype-based phylogenetic analysis and population genomics uncover the origin and domestication of sweetpotato
作者：Yan, Mengxiao;Wang, Yunze;Wang, Xinyi;Fan, Weijuan;Fang, Yijie;Wang, Yuqin;Nie, Haozhen;Wang, Hongxia;Yang, Jun;Li, Ming;Li, Ming;Wang, Yunze;Wang, Xinyi;Fang, Yijie;Wang, Yuqin;Moeinzadeh, M. -Hossein;Vingron, Martin;Quispe-Huamanquispe, Dora G.;Gheysen, Godelieve;Wang, Zhangying;Tanaka, Aiko;Heider, Bettina;Kreuze, Jan F.;Wang, Hongxia;Yang, Jun;Bock, Ralph
关键词：sweetpotato; origin; gene conversion; domestication; IbT-DNA; HPA
-
Effect of Miscellaneous Meals Replacing Soybean Meal in Feed on Growth Performance, Serum Biochemical Parameters, and Microbiota Composition of 25-50 kg Growing Pigs
作者：Zhan, Xianliang;Hou, Lei;He, Zhentao;Cao, Shuting;Wen, Xiaolu;Liu, Shuai;Li, Yaojie;Chen, Shaozhen;Zheng, Huayu;Deng, Dongyan;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wang, Li
关键词：miscellaneous meals; soybean meal; growth performance; microbiota
-
Accurate models and nutritional strategies for specific oxidative stress factors: Does the dose matter in swine production?
作者：Hong, Changming;Huang, Yujian;Cao, Shuting;Wang, Li;Yang, Xuefen;Hu, Shenglan;Gao, Kaiguo;Jiang, Zongyong;Xiao, Hao
关键词：Accurate models; Dose; Nutritional strategies; Oxidative stress; Swine
-
Maternal resveratrol improves the intestinal health and weight gain of suckling piglets during high summer temperatures: The involvement of exosome-derived microRNAs and immunoglobin in colostrum
作者：Hong, Changming;Huang, Yujian;Wen, Xiaolu;Wang, Li;Yang, Xuefen;Gao, Kaiguo;Jiang, Zongyong;Xiao, Hao;Hong, Changming;Huang, Yujian;Wen, Xiaolu;Wang, Li;Yang, Xuefen;Gao, Kaiguo;Jiang, Zongyong;Xiao, Hao;Hong, Changming;Yang, Guan
关键词：Colostrum; Exosome; Immunoglobulin; Intestinal health; Resveratrol
-
Fermented Corn-Soybean Meal Improved Growth Performance and Reduced Diarrhea Incidence by Modulating Intestinal Barrier Function and Gut Microbiota in Weaned Piglets
作者：Qiu, Yueqin;Tang, Jiaxi;Wang, Li;Yang, Xuefen;Jiang, Zongyong;Qiu, Yueqin;Tang, Jiaxi;Wang, Li;Yang, Xuefen;Jiang, Zongyong;Qiu, Yueqin;Tang, Jiaxi;Wang, Li;Yang, Xuefen;Jiang, Zongyong;Qiu, Yueqin;Tang, Jiaxi;Wang, Li;Yang, Xuefen;Jiang, Zongyong
关键词：fermented mixed feed; weaned piglets; growth performance; intestinal barrier; butyrate; gut microbiota
