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A Plant-Produced Recombinant Fusion Protein-Based Newcastle Disease Subunit Vaccine and Rapid Differential Diagnosis Platform

文献类型： 外文期刊

作者： Ma, Fanshu 1 ; Zhang, Erqin 2 ; Li, Qingmei 3 ; Xu, Qianru 1 ; Ou, Jiquan 4 ; Yin, Heng 4 ; Li, Kunpeng 4 ; Wang, Li 3 ; Zha 1 ;

作者机构： 1.Northwest A&F Univ, Coll Vet Med, Dept Prevent Vet Med, Yangling 712100, Shaanxi, Peoples R China

2.Henan Agr Univ, Coll Anim Sci & Vet Med, Dept Prevent Vet Med, Zhengzhou 450002, Peoples R China

3.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

4.Wuhan Healthgen Biotechnol Corp, Wuhan 430074, Peoples R China

关键词： Newcastle disease virus; fusion protein; rice endosperm; vaccine; plant-produced; DIVA; immunochromatographic strip

期刊名称：VACCINES （ 影响因子：4.422； 五年影响因子：5.513 ）

ISSN：

年卷期： 2020 年 8 卷 1 期

页码：

收录情况： SCI

摘要： Newcastle disease (ND) is a highly contagious avian disease, causing considerable economic losses to the poultry industry. To obtain a safe, inexpensive, and effective ND vaccine to meet the international trade requirements of differentiating infected from vaccinated animals (DIVA), here we report the production of Oryza sativa recombinant fusion (F) protein in stably transformed transgenic rice seeds via agroinfiltration. The F protein expression level was enhanced 3.6-fold with a genetic background in low glutelin. Inoculation of plant-produced F antigen into Specific Pathogen Free (SPF) chickens markedly elicited neutralizing antibody responses against homologous and heterologous ND virus strains. Two doses of 4.5 mu g fully protected chickens from a lethal ND challenge without any clinical symptoms. The mean weight gain of F protein-immunized chickens within 15 days after challenge was significantly higher than that of traditional whole virus vaccine-immunized chickens, thereby obtaining higher economic benefits. Moreover, the sera from the chickens vaccinated with the plant-produced F vaccine did not show reactivity in an immunochromatographic strip targeting the haemagglutinin-neuraminidase protein (HN) protein, and DIVA could be achieved within 10 minutes. Our results demonstrate that the plant-derived F vaccine along with immunochromatographic strips could be useful in the implementation of an NDV eradication program.

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