您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular and subcellular cryoinjury of fish spermatozoa and approaches to improve cryopreservation

文献类型： 外文期刊

作者： Xin Miaomiao 1 ; Niksirat, Hamid 1 ; Shaliutina-Kolesova, Anna 1 ; Siddique, Mohammad Abdul Momin 1 ; Sterba, Ja 1 ;

作者机构： 1.Univ South Bohemia Ceske Budejouvice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Zatisi 728-2, Vodnany 38925, Czech Republic

2.Chinese Acad Fishery Sci, Sino Czech Joint Lab Fish Conservat & Biotechnol, Key Lab Freshwater Biodivers Conservat, Minist Agr China,Yangtze River Fisheries Res Inst, Wuhan, Peoples R China

3.Noakhali Sci & Technol Univ, Dept Oceanog, Noakhali, Bangladesh

4.Univ South Bohemia Ceske Budejouvice, Inst Chem & Biochem, Fac Sci, Ceske Budejouvice, Czech Republic

5.Acad Sci Czech Republ, Ctr Biol, Inst Parasitol, Ceske Budejouvice, Czech Republic

关键词： antifreeze glycoproteins; antifreeze proteins; antioxidants; cryodamage; seminal plasma; spermatozoa

期刊名称：REVIEWS IN AQUACULTURE （ 影响因子：10.592； 五年影响因子：10.455 ）

ISSN： 1753-5123

年卷期： 2020 年 12 卷 2 期

页码：

收录情况： SCI

摘要： The quality of frozen/thawed fish sperm is generally lower than that of fresh sperm. Extremely low temperatures are associated with damage to fish spermatozoon subcellular compartments and associated molecules such as DNA and proteins. Cryodamage can negatively affect DNA integrity, spermatozoon metabolism and motility, consequently impairing fertilization and embryo development. To preserve sperm efficiently, the addition of proteins including seminal plasma proteins, antioxidants, antifreeze proteins and antifreeze glycoproteins, and bovine serum albumin before and/or after freezing is suggested to minimize cryoinjury. An appropriate quantity of seminal plasma components in cryopreservation medium can prevent spermatozoon membrane damage by maintaining antioxidant enzymes and their proper distribution on the spermatozoon surface. Under storage conditions, the semen antioxidant system is ineffective in protecting spermatozoa from reactive oxygen species. The addition of antioxidants can provide protection for spermatozoa and reduce cryoinjuries during storage, but the effect of a given antioxidant is species-specific. Antifreeze proteins and antifreeze glycoproteins can stabilize the cell membrane via interaction with phospholipid components and inhibit ice-crystal growth to reduce damage related to osmotic stress and ice crystallization. However, they may exhibit a cytotoxic effect. This review summarizes the sources and characteristics of subcellular spermatozoon cryoinjury and discusses approaches to improve outcome of fish sperm cryopreservation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台