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AQUA_2020_1080: Sperm management of European catfish (Silurus glanis L.) for effective reproduction and genetic conservation

文献类型： 外文期刊

作者： Linhart, Otomar 1 ; Cheng, Yu 1 ; Rodina, Marek 1 ; Gela, David 1 ; Tuckova, Vladimira 1 ; Shelton, William L. 1 ; Tin 1 ;

作者机构： 1.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Zatisi 728-2, Vodnany 38925, Czech Republic

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Sino Czech Joint Lab Fish Conservat & Biotechnol, Wuhan, Peoples R China

3.Istanbul Univ, Fac Aquat Sci, Ordu Str 08, TR-34134 Istanbul, Turkey

关键词： Egg; Fertilization; Fish reproduction; Sperm storage; Silurus glanis; Spermatozoa

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2020 年 529 卷

页码：

收录情况： SCI

摘要： The European catfish (Silurus glanis; also called wels or sheatfish) is one of the largest commercially important freshwater fish species. The objective of this study was to examine the storage time of its semen in an immobilization solution (IS) to enhance conservation and aquaculture programs. Changes in spermatozoan motility was examined in semen stored on ice in 20 mL tubes in IS for 0, 1, 7, 14 and 28 days post-stripping (DPS). Stored sperm samples were pooled and used for fertility testing at 1 and 7 DPS. The IS maintained sperm motility at > 90% during days 1-7 of semen storage, and curvilinear velocity (VCL) and straight-line velocity (VSL) were up to the levels of 163 and 90 mu m s(-1), respectively. Spermatozoa stored for 14 days had 60% motility and VCL and VSL values were similar to those of 7 DPS. Sperm motility was only 1% at 28 DPS with VCL and VSL of 79 and 24 mu m s(-1), respectively. The fertilization and total hatching rates of eggs activated with sperm stored for 7 days were reduced, but the slight decrease was compensated by the increase in supply of spermatozoa which resulted in overall 80% level of fertility and hatching. The number of larval malformations was not correlated with storage time of sperm. Based on the results of this study, sperm from the European catfish can be collected and stored in IS for as long as 1 week, retaining quality sufficient for fertilization to yield normal fry.

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