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Ectopic expression of HaNAC1, an ATAF transcription factor from Haloxylon ammodendron, improves growth and drought tolerance in transgenic Arabidopsis

文献类型： 外文期刊

作者： Gong, Lei 1 ; Zhang, Haiwen 2 ; Liu, Xuan 1 ; Gan, Xiaoyan 1 ; Nie, Fengjie 1 ; Yang, Wenjing 1 ; Zhang, Li 1 ; Chen, Yuc 1 ;

作者机构： 1.Ningxia Acad Agr & Forestry Sci, Agr Biotechnol Ctr, Ningxia Key Lab Agrobiotechnol, 590 Huanghe East Rd, Yinchuan 750002, Ningxia Hui Nat, Peoples R China

2.Ningxia Univ, Sch Life Sci, 489 Helanshan West Rd, Yinchuan 750021, Ningxia Hui Nat, Peoples R China

3.Ludong Univ, Coll Agr, 186 Hongqizhong Rd, Yantai 264025, Peoples R China

4.Ludong Univ, Key Lab Mol Module Based Breeding High Yield & Ab, 186 Hongqizhong Rd, Yantai 264025, Shandong, Peoples R China

关键词： NAC transcription Factor; HaNAC1; Drought stress; Haloxylon ammodendron

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 151 卷

页码：

收录情况： SCI

摘要： NAC transcription factors play a pivotal role in plant growth, development and response to abiotic stress. However, their biological functions in desert trees are largely unknown. In this work, the NAC transcription factor HaNAC1 from Haloxylon ammodendron, a typical wooden plant normally grown in desert, was isolated, and its possible role in plant growth and resistance to drought stress was investigated. HaNAC1 encodes an ATAF subfamily transcription factor containing one NAC domain with five conserved regions. Quantitative real time PCR analyses revealed that HaNAC1 was ubiquitously expressed in various tissues and organs such as roots, stems, leaves and seeds, with a predominant expression in stems. Further studies demonstrated that expression of HaNAC1 was significantly induced by osmotic stress in Haloxylon ammodendron seedlings, and subcellular localization analysis indicated that GFP-HaNAC1 fusion protein was localized to the nucleus in Arabidopsis leaf protoplast. Ectopic expression of HaNAC1 led to promoted growth and drought tolerance in transgenic Arabidopsis, accompanied with up-regulated expression of stress-inducible marker genes, and increased accumulation of proline, IAA and ABA under both normal and drought stress conditions. In addition, co-immunoprecipitation and Bi-molecular fluorescence complementation assays illustrated that HaNAC1 directly interacted with AtNAC32. All these results suggest that HaNAC1 is involved in both the growth and drought resistance of Haloxylon ammodendron, and could be used as a promising candidate gene for the breeding of crops with augmented tolerance to drought stress.

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