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Regenerative plantlets with improved agronomic characteristics caused by anther culture of tetraploid potato (Solanum tuberosum L.)

文献类型： 外文期刊

作者： Zhang, Li 1 ; Nie, Feng-jie 1 ; Gong, Lei 1 ; Gan, Xiao-yan 1 ; Zhang, Guo-hui 3 ; Liu, Xuan 1 ; Yang, Wen-jing 1 ; Shi, Lei 1 ; Chen, Yu-chao 1 ; Xie, Rui-xia 3 ; Guo, Zhi-qian 3 ; Song, Yuxia 1 ;

作者机构： 1.Ningxia Acad Agr & Forestry Sci, Res Ctr Agr Biotechnol, Yinchuan, Ningxia, Peoples R China

2.Gansu Agr Univ, Coll Agr, Lanzhou, Gansu, Peoples R China

3.Ningxia Acad Agr & Forestry Sci, Guyuan Inst Agr Sci, Guyuan, Ningxia, Peoples R China

关键词： Potato (Solanum tuberosum L; ); Anther culture; Callus; Regenerative; Ploidy; Morphological identification

期刊名称：PEERJ （ 影响因子：2.7； 五年影响因子：3.1 ）

ISSN： 2167-8359

年卷期： 2023 年 11 卷

页码：

收录情况： SCI

摘要： Objective. As the primary means of plant-induced haploid, anther culture is of great significance in quickly obtaining pure lines and significantly shortening the potato breeding cycle. Nevertheless, the methods of anther culture of tetraploid potato were still not well established. Methods. In this study, 16 potato cultivars (lines) were used for anther culture in vitro. The corresponding relation between the different development stages of microspores and the external morphology of buds was investigated. A highly-efficient anther culture system of tetraploid potatoes was established. Results. It was shown in the results that the combined use of 0.5 mg/L 1-Naphthylacetic acid (NAA), 1.0 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D), and 1.0 mg/L Kinetin (KT) was the ideal choice of hormone pairing for anther callus. Ten of the 16 potato cultivars examined could be induced callus with their respective anthers, and the induction rate ranged from 4.44% to 22.67% using this hormone combination. According to the outcome from the orthogonal design experiments of four kinds of appendages, we found that the medium with sucrose (40 g/L), AgNO3 (30 mg/L), activated carbon (3 g/L), potato extract (200 g/L) had a promotive induction effect on the anther callus. In contrast, adding 1 mg/L Zeatin (ZT) effectively facilitated callus differentiation. Conclusion. Finally, 201 anther culture plantlets were differentiated from 10 potato cultivars. Among these, Qingshu 168 and Ningshu 15 had higher efficiency than anther culture. After identification by flow cytometry and fluorescence in situ hybridization, 10 haploid plantlets (5%), 177 tetraploids (88%), and 14 octoploids (7%) were obtained. Some premium anther-cultured plantlets were further selected by morphological and agronomic comparison. Our findings provide important guidance for potato ploidy breeding.

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