A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres
文献类型： 外文期刊
作者： Liu, Xiao 1 ; Yang, Jifei 2 ; Li, Qingmei 2 ; Wang, Yinbiao 4 ; Wang, Yanhong 2 ; Li, Ge 1 ; Shi, Jianzhou 2 ; Ding, Peiy 1 ;
作者机构： 1.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China
2.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
3.Henan Med Coll, Dept Med, Zhengzhou 451191, Peoples R China
4.Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Henan, Peoples R China
5.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China
关键词： Lateral flow immunoassay; Nanocomposite; Influenza virus; Point-of-care test; Visual determination
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
ISSN： 0026-3672
年卷期： 2020 年 187 卷 5 期
页码：
收录情况： SCI
摘要： A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The AuNP and PS particles were linked using monoclonal antibodies against influenza virus as the bridge. Under the optimal conditions, the visual detection limit of the AuNP-PS-based strip test was as low as 1/16 hemagglutination unit (HAU). It was 64 times higher than that of 10 nm (4 HAU) AuNP-based strip tests. Quantitative analysis showed that the detection limit of the AuNP-PS-based strip is 0.016 HAU. The AuNP-PS-based strip test showed no cross-reactivity to the other subtypes (H1, H5, H7, or H9) of influenza viruses. .
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