A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres
文献类型： 外文期刊
作者： Liu, Xiao 1 ; Yang, Jifei 2 ; Li, Qingmei 2 ; Wang, Yinbiao 4 ; Wang, Yanhong 2 ; Li, Ge 1 ; Shi, Jianzhou 2 ; Ding, Peiy 1 ;
作者机构： 1.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China
2.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
3.Henan Med Coll, Dept Med, Zhengzhou 451191, Peoples R China
4.Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Henan, Peoples R China
5.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China
关键词： Lateral flow immunoassay; Nanocomposite; Influenza virus; Point-of-care test; Visual determination
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
ISSN： 0026-3672
年卷期： 2020 年 187 卷 5 期
页码：
收录情况： SCI
摘要： A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The AuNP and PS particles were linked using monoclonal antibodies against influenza virus as the bridge. Under the optimal conditions, the visual detection limit of the AuNP-PS-based strip test was as low as 1/16 hemagglutination unit (HAU). It was 64 times higher than that of 10 nm (4 HAU) AuNP-based strip tests. Quantitative analysis showed that the detection limit of the AuNP-PS-based strip is 0.016 HAU. The AuNP-PS-based strip test showed no cross-reactivity to the other subtypes (H1, H5, H7, or H9) of influenza viruses. .
- 相关文献
作者其他论文 更多>>
-
Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation
作者：Qian, Qisheng;Zhang, Gaiping;Qian, Qisheng;Zhao, Shuang-shuang;Yang, Lei;Xing, Guangxu;Li, Rui;Qiao, Songlin;Zhang, Gaiping;Qian, Qisheng;Zhang, Gaiping;Chen, Yumei;Liang, Chao;Wang, Haili;Wang, Aiping
关键词：porcine epidemic diarrhea virus; spike protein; palmitoylation; protein stability; chaperone-mediated autophagy
-
Fine mapping of conserved neutralizing epitopes within the VP2 protein of Senecavirus A using monoclonal antibodies
作者：Zou, Wanying;Li, Chunzhen;Meng, Zekun;Zhang, Gaiping;Zou, Wanying;Li, Qingmei;Li, Chunzhen;Meng, Zekun;Sun, Yaning;Yang, Suzhen;Guo, Junqing;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：Senecavirus A; VP2 protein; Monoclonal antibodies; Neutralizing epitope
-
Identification of linear B-cell epitopes of Senecavirus A VP2 protein using monoclonal antibodies
作者：Jiang, Yao;Zhang, Gaiping;Guo, Zhenhua;Weng, Maoyang;Chen, Linlin;Li, Qingmei;Qiao, Songlin;Zhang, Gaiping;Zhang, Lei;Zhang, Gaiping;Zhang, Gaiping
关键词：Senecavirus A; VP2 protein; monoclonal antibodies; B-cell epitopes; spatial structure
-
Detection method for identifying duck hepatitis A virus 3 virulent and attenuated strains based on RPA CRISPR single-base recognition system
作者：Chen, Lei;Zhang, Qiaoli;Sun, Wenbo;Mauk, Michael G.;Li, Qingmei
关键词：DHAV-3 virulent and attenuated strains; RPA-CRISPR; Single-base recognition
-
PEDV Nsp14 induces mitophagy-mediated degradation of MAVS to antagonize host innate immunity and facilitate viral proliferation
作者：Yang, Lei;Zhang, Gaiping;Yang, Lei;Qian, Qisheng;Chen, Xin-xin;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Yang, Lei;Zhang, Gaiping;Sun, Yan-gang;Zhang, Jia-qing;Xing, Bao-song
关键词：PEDV; Nsp14; mitophagy; IFN-beta; NDP52
-
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides
作者：Li, Qingmei;Wang, Ruining;Yang, Jifei;Guo, Junqing;Zhang, Gaiping;Li, Ge;Wang, Xun;Zhang, Gaiping;Wang, Ruining;Zhang, Gaiping
关键词：Fc-binding epitope; BoFc gamma RI; Bovine IgG1; Synthetic peptides; Binding inhibition
-
Genomic characterization and evolutionary analysis of a Getah virus variant from piglets in central China
作者：Guo, Zhenhua;Zhang, Gaiping;Jiang, Yao;Zhang, Gaiping;Li, Peng;Zhang, Gaiping;Zhang, Gaiping
关键词：Getah virus; genomic characteristics; genetic evolution; E2 protein; positive selection
