Green Tea Polyphenol EGCG Attenuates MDSCs-mediated Immunosuppression through Canonical and Non-Canonical Pathways in a 4T1 Murine Breast Cancer Model
文献类型： 外文期刊
作者： Xu, Ping 1 ; Yan, Feng 2 ; Zhao, Yueling 1 ; Chen, Xiangbo 3 ; Sun, Shili 4 ; Wang, Yuefei 1 ; Ying, Le 1 ;
作者机构： 1.Zhejiang Univ, Dept Tea Sci, Hangzhou 310058, Peoples R China
2.Monash Univ, Australian Ctr Blood Dis, Cent Clin Sch, Melbourne, Vic 3004, Australia
3.Northeast Normal Univ, Minist Educ, Key Lab Mol Epigenet, Changchun 130024, Peoples R China
4.Guangdong Acad Agr Sci, Tea Res Inst, Guangzhou 510640, Peoples R China
5.Hudson Inst Med Res, Ctr Innate Immun & Infect Dis, Clayton, Vic 3168, Australia
关键词： EGCG; anti-tumor mechanism; MDSCs; immunosuppression; non-canonical pathways
期刊名称：NUTRIENTS （ 影响因子：5.717； 五年影响因子：6.349 ）
ISSN：
年卷期： 2020 年 12 卷 4 期
页码：
收录情况： SCI
摘要： Several studies in the past decades have reported anti-tumor activity of the bioactive compounds extracted from tea leaves, with a focus on the compound epigallocatechin-3-gallate (EGCG). However, further investigations are required to unravel the underlying mechanisms behind the anti-tumor activity of EGCG. In this study, we demonstrate that EGCG significantly inhibits the growth of 4T1 breast cancer cells in vitro and in vivo. EGCG ameliorated immunosuppression by significantly decreasing the accumulation of myeloid-derived suppressor cells (MDSCs) and increasing the proportions of CD4(+) and CD8(+) T cells in spleen and tumor sites in 4T1 breast tumor-bearing mice. Surprisingly, a low dose of EGCG (0.5-5 mu g/mL) effectively reduced the cell viability and increased the apoptosis rate of MDSCs in vitro. EGCG down-regulated the canonical pathways in MDSCs, mainly through the Arg-1/iNOS/Nox2/NF-kappa B/STAT3 signaling pathway. Moreover, transcriptomic analysis suggested that EGCG also affected the non-canonical pathways in MDSCs, such as ECM-receptor interaction and focal adhesion. qRT-PCR further validated that EGCG restored nine key genes in MDSCs, including Cxcl3, Vcan, Col4a1, Col8a1, Oasl2, Mmp12, Met, Itsnl and Acot1. Our results provide new insight into the mechanism of EGCG-associated key pathways/genes in MDSCs in the murine breast tumor model.
- 相关文献
作者其他论文 更多>>
-
Mechanism of Green Tea Peptides in Lowering Blood Pressure and Alleviating Renal Injury Induced by Hypertension Through the Ang II/TGF-β1/SMAD Signaling Pathway
作者：Li, Lulu;Li, Dongli;Li, Lulu;Sun, Shili;Lai, Xingfei;Li, Qiuhua;Chen, Ruohong;Zhang, Zhenbiao;Hao, Mengjiao;Zhang, Suwan;Sun, Lingli;Li, Dongli
关键词：tea polypeptide; hypertension; renal injury; TGF-beta/Smad signaling
-
Metabolomics-based analysis of the effects of differences in soluble sugars on the sweetness quality of six major tea types in China
作者：Li, Wen;Chen, Zhongzheng;Zhang, Zhenbiao;Chen, Ruohong;Sun, Lingli;Lai, Xingfei;Li, Qiuhua;Hao, Mengjiao;Zhang, Suwang;Sun, Shili;Li, Qian
关键词：
-
Mechanistic study of heat-shock regulation of endogenous protease activity in paddy rice to improve the taste quality of parboiled rice
作者：Yan, Feng;Liu, Ping;Tong, Shisheng;Bai, Song
关键词：Heat shock; Parboiled rice; Endogenous protease; Taste quality
-
Effects of Pile-Fermentation Duration on the Taste Quality of Single-Cultivar Large-Leaf Dark Tea: Insights from Metabolomics and Microbiomics
作者：Yang, Wanying;Ni, He;Chen, Ruohong;Sun, Lingli;Li, Qiuhua;Lai, Xingfei;Zhang, Zhenbiao;Lai, Zhaoxiang;Hao, Mengjiao;Sun, Shili;Li, Qian;Lin, Sen
关键词：dark tea; quality; taste; pile-fermentation; metabolomics; microbiomics
-
Analysis of volatile metabolites and differences in aroma quality of green tea, black tea and oolong tea prepared from highly aromatic tea variety (Dancong)
作者：Zhang, Suwan;Xia, Hongling;Sun, Shili;Ma, Chenying;Pan, Shunshun;Zhang, Zhenbiao;Lai, Xingfei;Li, Qiuhua;Hao, Mengjiao;Sun, Lingli;Chen, Ruohong;Zhang, Suwan;Xia, Hongling;Sun, Shili;Ma, Chenying;Pan, Shunshun;Zhang, Zhenbiao;Lai, Xingfei;Li, Qiuhua;Hao, Mengjiao;Sun, Lingli;Chen, Ruohong;Li, Feng;Ma, Chenying
关键词：Fermentation degree; Tea; Aroma; VOCs; GC-MS
-
Inhibition of xanthine oxidase by copper-gallate coordination polymers and its mechanistic study
作者：Mo, Yejian;Ren, Jian;Sun, Shili;Shen, Xiaoyu;Mo, Yejian;Ren, Jian;Liu, Kexin;Deng, Chunlin;Shen, Xiaoyu;Mo, Yejian;Ren, Jian;Liu, Kexin;Deng, Chunlin
关键词：Hyperuricemia; Metal-polyphenol coordination polymers; Xanthine oxidase inhibitors; Antioxidant properties
-
Low-Dose, Post-Storage Dancong Tea Attenuates Hydroalcohol-Induced Gastric Damage via Modulation Antioxidant and Anti-Inflammatory Pathways
作者：Jian, Huanwei;Chen, Zhongzheng;Chen, Ruohong;Sun, Lingli;Li, Qiuhua;Cao, Junxi;Lai, Xingfei;Zhang, Zhenbiao;Zhang, Suwan;Hao, Mengjiao;Sun, Shili
关键词：gastric injury; new tea; stored tea; low-dose; inflammatory; antioxidant
