Agrobacterium tumefaciens-mediated transformation of a hevein-like gene into asparagus leads to stem wilt resistance
文献类型： 外文期刊
作者： Chen, Helong 1 ; Guo, Anping 1 ; Lu, Zhiwei 4 ; Tan, Shibei 3 ; Wang, Jian 1 ; Gao, Jianming 2 ; Zhang, Shiqing 2 ; Huang 1 ;
作者机构： 1.Hainan Univ, Inst Trop Agr & Forestry, Haikou, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr,Hainan Acad Trop Agr Resource, Haikou, Hainan, Peoples R China
3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China
4.Chinese Acad Trop Agr Sci, South Subtrop Crops Inst, Zhanjiang City Key Lab Trop Crops Genet Improveme, Zhanjiang, Guangdong, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2019 年 14 卷 10 期
页码：
收录情况： SCI
摘要： Asparagus stem wilt, is a significant and devastating disease, typically leading to extensive economic losses in the asparagus industry. To obtain transgenic plants resistant to stem wilt, the hevein-like gene, providing broad spectrum bacterial resistance was inserted into the asparagus genome through Agrobacterium tumefaciens-mediated transformation. The optimal genetic transformation system for asparagus was as follows: pre-culture of embryos for 2 days, inoculation using a bacterial titre of OD600 = 0.6, infection time 10 min and co-culturing for 4 days using an Acetosyringone concentration of 200 mu mol/L. Highest transformation frequencies reached 21% and ten transgenic asparagus seedlings carrying the hevein-like gene were identified by polymerase chain reaction. Moreover, integration of the hevein-like gene in the T1 generation of transgenic plants was confirmed by southern blot hybridization. Analysis showed that resistance to stem wilt was enhanced significantly in the transgenic plants, in comparison to non-transgenic plants. The results provide additional data for genetic improvement and are of importance for the development of new disease-resistant asparagus varieties.
- 相关文献
作者其他论文 更多>>
-
Physiological activities, transcriptomes and metabolomes of Pyropia yezoensis conchocelis unveil the roles of pyPGK, pyBCKDHA, and pyDLD in response to freshwater soaking
作者：Huang, Xueying;Li, Feng;He, Linwen;Huang, Xueying;Wang, Yu;Zhao, Hui;Li, Huiliang;Gu, Fenglin;Tan, Deguan;Hu, Wei;Guo, Anping;Ji, Changmian;Huang, Xueying;Wang, Yu;Zhao, Hui;Li, Huiliang;Gu, Fenglin;Tan, Deguan;Hu, Wei;Guo, Anping;Ji, Changmian;Wang, Yu;Zeng, Liwang
关键词：Pyropia yezoensis; Freshwater stress; Physiological activities; Transcriptome; Metabolome
-
Population Structure and Genetic Diversity of Agave Germplasms in China
作者：Hu, Xiaoli;Li, Yubo;Tan, Shibei;Mkapa, Dietram Samson;Huang, Xing;Hu, Xiaoli;Mkapa, Dietram Samson;Li, Yubo;Chen, Lisha;Mkapa, Dietram Samson;Lin, Chen;Liu, Qingqing;Jin, Gang;Chen, Tao;Qin, Xu;Yi, Kexian;Huang, Xing;Huang, Xing
关键词：agave germplasms; population structure; genetic diversity; genetic differentiation; amplicon sequencing; SNP; phylogeny
-
Identification and potential of the hyperparasite Acremonium persicinum as biocontrol agent against coffee leaf rust
作者：Liang, Minli;Liang, Yanqiong;Huang, Xing;Lu, Ying;Wang, Bo;Tan, Shibei;Chen, Helong;He, Chunping;Yi, Kexian;Wu, Weihuai;Bai, Xuehui;Chen, Zhenjia;Yi, Kexian
关键词：Acremonium persicinum; coffee leaf rust (CLR); Hemileia vastatrix; hyperparasitism
-
Thiolutin Is a Potential Fungicide for Controlling Phytophthora nicotianae and Its Mechanistic Study
作者：Xu, Gang;Huang, Xing;Liang, Yanqiong;Tan, Shibei;Chen, Helong;Yi, Kexian;Wu, Weihuai;Xu, Gang;Ma, Xiang;Xiong, Zijun;Guo, Zhikai;Xiong, Zijun;Guo, Zhikai
关键词：
Phytophthora nicotianae ; thiolutin; antifungal activity; mitochondria bursting -
Agave macroacantha Transcriptome Reveals Candidate CNGC Genes Responsive to Cold Stress in Agave
作者：Li, Yubo;Zhang, Weiyi;Li, Yubo;Hu, Xiaoli;Mkapa, Dietram Samson;Tan, Shibei;Chen, Helong;Huang, Xing;Hu, Xiaoli;Mkapa, Dietram Samson;Mkapa, Dietram Samson;Xie, Li;Guo, Pingan;Huang, Xing;Huang, Xing;Yi, Kexian
关键词：
Agave macroacantha ; transcriptome;CNGC genes; expression; cold stress -
Metabolome and transcriptome analysis reveal the pigments biosynthesis pathways in different color fruit peels of Clausena lansium L. Skeels
作者：Zhao, Zhichang;Luo, Ruixiong;Yu, Huaping;Pang, Yali;Wang, Jian;Gao, Aiping;Adjei, Mark Owusu;Ma, Jun;Zhang, Yu
关键词：
Clausena lansium L.Skeels ; fruits peels; anthocyanins; flavonoids; fruit peel color -
Investigating the impact of soil properties on the nutrient content and microbial community of sisal (Agave sisalana Perrine)
作者：Liu, Ping;Huang, Zhaoxue;Yi, Kexian;Chen, Helong;Liu, Ping;Huang, Zhaoxue;Tan, Shibei;Wu, Weihuai;Yi, Kexian;Chen, Helong;Chen, Tao;Qin, Jianfeng;Yi, Kexian
关键词：High-throughput sequencing; plant nutrient content; rhizosphere soil microorganisms; sisal; soil pH.
