您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Selection of suitable reference genes for qRT-PCR expression analysis of Codonopsis pilosula under different experimental conditions

文献类型： 外文期刊

作者： Yang, Jing 1 ; Yang, Xiaozeng 2 ; Kuang, Zheng 2 ; Li, Bin 1 ; Lu, Xiayang 1 ; Cao, Xiaoyan 1 ; Kang, Jiefang 1 ;

作者机构： 1.Shaanxi Normal Univ, Key Lab, Natl Engn Lab Resource Dev Endangered Crude Drugs, Minist Educ Med Resources & Nat Pharmaceut Chem, Xian 710062, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China

3.Peking Univ, Sch Life Sci, Peking Tsinghua Ctr Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

4.Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China

关键词： Codonopsis pilosula; Developmental stages; Tissues; Stress treatments; qRT-PCR; Reference genes

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2020 年 47 卷 6 期

页码：

收录情况： SCI

摘要： Codonopsis pilosula is a well-known medicinal plant. Although its transcriptome sequence has been published, suitable reference genes have not been systematically identified for conducting expression analyses via quantitative real-time polymerase chain reaction (qRT-PCR). To screen appropriate genes for use with this species, we applied four different methods-GeNorm, NormFinder, BestKeeper, and RefFinder-to evaluate the stability of 13 candidates: CpiEF1Bb, CpiCACS, CpiF-Box, Cpi beta-Tubulin, CpiGAPDH, CpiActin2, CpiAPT1, CpiActin7, CpiActin8, CpiRPL6, CpiHAF1, CpiTubulin6, and CpiUBQ12. Expression was examined by qRT-PCR for various tissue types, chemical treatments, and developmental stages. For all tested samples, CpiGAPDH proved to be the most stable. Comprehensive analysis indicated that the most stable internal reference genes were CpiF-Box and CpiCACS in different tissues and at different developmental stages, respectively. Under NaCl stress, CpiAPT1 was the best internal reference gene. For methyl jasmonate and abscisic acid treatments, CpiGAPDH and CpiF-Box, respectively, presented the highest degree of expression stability. Based on these findings, we chose CpiSPL9 as the target gene for validating the suitability of these selected reference genes. All of these results provide a foundation for accurate quantification of expression levels by genes of interest in C. pilosula.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台