Quantification of zearalenone in mildewing cereal crops using an innovative photoelectrochemical aptamer sensing strategy based on ZnO-NGQDs composites
文献类型： 外文期刊
作者： Luo, Lijun 1 ; Liu, Xiaohong 1 ; Ma, Shuai 1 ; Li, Libo 1 ; You, Tianyan 1 ;
作者机构： 1.Jiangsu Univ, Sch Agr Equipment Engn, Key Lab Modern Agr Equipment & Technol, Minist Educ,High Tech Key Lab Agr Equipment & Int, Zhenjiang 212013, Jiangsu, Peoples R China
2.Univ Penn, Sch Engn & Appl Sci, Dept Bioengn, Philadelphia, PA 19104 USA
3.Minist Agr, Risk Assessment Lab AgroProd Beijing, Beijing Municipal Key Lab Agr Environm Monitoring, Beijing Res Ctr Agr Stand & Testing, 9 Middle Rd Shu Guang Hua Yuan, Beijing 100097, Peoples R China
关键词： Mildew process monitoring; Photoelectrochemical aptasensor; Zinc oxide-nitrogen doped graphene quantum dots; Zearalenone
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2020 年 322 卷
页码：
收录情况： SCI
摘要： Zearalenone (ZEN) is highly toxic to humans, and therefore, development of sensitive and effective methods for ZEN quantification in cereal crops is particularly important. Here, an innovative photoelectrochemical (PEC) aptasensor based on simply in-situ conjugated composites of zinc oxide-nitrogen doped graphene quantum dots (ZnO-NGQDs) was constructed. On addition of NGQDs, the composites displayed higher PEC signal with 8.8-fold enhancement than pure ZnO nanoparticles. A sensitive and selective PEC aptasensor was fabricated by combining the composites with ZEN aptamer, which yielded an excellent analytical performance for ZEN detection, with a wide linear range of 1.0 x 10(-13)-1.0 x 10(-7) g mL(-1) and a low detection limit of 3.3 x 10(-14) g mL(-1). Good recoveries were obtained using the PEC aptasensor, which were consistent with those obtained using the national standard method (HPLC-MS). Finally, ZEN in mildewing cereal crops was monitored with the PEC aptasensor, exhibiting good potential for application in cereal crops for early diagnosis.
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