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Rapid on-site detection of genetically modified soybean products by real-time loop-mediated isothermal amplification coupled with a designed portable amplifier

文献类型： 外文期刊

作者： Wu, Hui 1 ; Zhang, Xianjun 3 ; Wu, Baoqiang 3 ; Qian, Cheng 1 ; Zhang, Fang 4 ; Wang, Liu 2 ; Ye, Zunzhong 1 ; Wu, Jian 1 ;

作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, State Key Lab Qual & Safety Agroprod, Hangzhou 310021, Peoples R China

3.Hangzhou An Yu Technol Co Ltd, Hangzhou 310021, Peoples R China

4.Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China

关键词： Genetically modified; Soybean products; On-site detection; Loop-mediated isothermal amplification; Portable instrument

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2020 年 323 卷

页码：

收录情况： SCI

摘要： Herein, we developed a rapid method for detection of genetically modified soybean (GTS 40-3-2) products using loop-mediated isothermal amplification (LAMP). A crude 5-minute extraction method was established for DNA extraction from soybeans and soybean products. LAMP reaction for CaMV35S promoter was optimized and the fastest threshold time (Tt) was 14 min with 4 mM magnesium ions at 63 degrees C. A portable instrument was designed to perform real-time LAMP in the field. As little as 0.1% GM soybean, specifically, or 0.5% GM ingredients in Chinese traditional tofu could be detected in 30 min from sampling to results. We used this method to further assess other five soybean products to determine whether they contained transgenic ingredients and compared the results with those obtained using PCR, which suggested the proposed method was applicable for rapid detection of genetically modified soybean in food products.

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