A rapid and convenient method for on-site detection of MON863 maize through real-time fluorescence recombinase polymerase amplification
文献类型： 外文期刊
作者： Wang, Xiaofu 1 ; Xie, Shilong 1 ; Chen, Xiaoyun 1 ; Peng, Cheng 1 ; Xu, Xiaoli 1 ; Wei, Wei 1 ; Ma, Tongfu 3 ; Cai, Jian; 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Qual & Safety Agroprod, Hangzhou 310021, Peoples R China
2.Minist Agr China, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Peoples R China
3.Fuyang Normal Univ, Biol & Food Engn Sch, Fuyang 236037, Peoples R China
关键词： Genetically modified crop; Recombinase polymerase amplification (RPA); Real-time fluorescence; On-site detection; MON863
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2020 年 324 卷
页码：
收录情况： SCI
摘要： As large-scale planting of genetically modified (GM) crops increases, the development of a rapid and convenient method for on-site detection of GM crops is important. We combined the advantages of recombinase polymerase amplification (RPA) and fluorescence detection to establish a rapid, sensitive, specific, and simple detection platform for on-site detection of MON863 maize. Test samples were added directly to the platform after simple pre-treatment with a DNA extraction-free method. Results were obtained through real-time monitoring with a portable instrument, which facilitated sample-in/answer-out on-site detection. The entire detection process, including sample preparation, RPA and identification of amplification results, was accomplished in approximately 10 min. Furthermore, the detection was achieved with a simple and inexpensive portable device. This method has high potential for application in other fields requiring rapid detection of DNA targets, such as in field research, resource-limited areas, and science education.
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