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Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation

文献类型： 外文期刊

作者： Liu, Lulu 1 ; Wang, Yu 1 ; Liang, Xiaojuan 1 ; Wu, Xiao 4 ; Liu, Jiali 1 ; Yang, Shulin 1 ; Tao, Cong 1 ; Zhang, Jin 5 ; Tia 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Lab Anim Poultry Genet Breeding & Reprod, Minist Agr, Beijing 100193, Peoples R China

2.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

3.Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China

4.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

5.Jiaxing Univ, Coll Biol Chem Sci & Engn, Jiaxing 314001, Peoples R China

6.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China

7.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China

关键词： SCD; adipocytes; transdifferentiation; adipogenesis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 7 期

页码：

收录情况： SCI

摘要： Fat deposition, which influences pork production, meat quality and growth efficiency, is an economically important trait in pigs. Numerous studies have demonstrated that stearoyl-CoA desaturase (SCD), a key enzyme that catalyzes the conversion of saturated fatty acids into monounsaturated fatty acids, is associated with fatty acid composition in pigs. As SCD was observed to be significantly induced in 3T3-L1 preadipocytes differentiation, we hypothesized that it plays a role in porcine adipocyte differentiation and fat deposition. In this study, we revealed that SCD is highly expressed in adipose tissues from seven-day-old piglets, compared to its expression in tissues from four-month-old adult pigs. Moreover, we found that SCD and lipogenesis-related genes were induced significantly in differentiated porcine adipocytes. Using CRISPR/Cas9 technology, we generated SCD-/- porcine embryonic fibroblasts (PEFs) and found that the loss of SCD led to dramatically decreased transdifferentiation efficiency, as evidenced by the decreased expression of known lipid synthesis-related genes, lower levels of oil red O staining and significantly lower levels of triglyceride content. Our study demonstrates the critical role of SCD expression in porcine adipocyte differentiation and paves the way for identifying it as the promising candidate gene for less fat deposition in pigs.

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