Fine Mapping a Broad-Spectrum Powdery Mildew Resistance Gene in Chinese Landrace Datoumai, PmDTM, and Its Relationship with Pm24
文献类型： 外文期刊
作者： Lu, Nan 1 ; Lu, Mingxue 1 ; Liu, Pan 1 ; Xu, Hongxing 1 ; Qiu, Xiaolong 1 ; Hu, Shanshan 1 ; Wu, Yanan 1 ; Bai, Shenglong 1 ;
作者机构： 1.Henan Univ, Sch Life Sci, State Key Lab Cotton Biol, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Henan, Peoples R China
2.Nanjing Agr Univ, Crop Genom & Bioinformat Ctr, Appl Plant Genom Lab, Nanjing 210095, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Nanjing 210014, Jiangsu, Peoples R China
关键词： Blumeria graminis f. sp. tritici; candidate gene; fine mapping; powdery mildew; Pm24; wheat
期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）
ISSN： 0191-2917
年卷期： 2020 年 104 卷 6 期
页码：
收录情况： SCI
摘要： Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a globally important wheat disease causing severe yield losses, and deployment of resistant varieties is the preferred choice for managing this disease. Chinese wheat landrace Datoumai was resistant to 22 of 23 Bgt isolates at the seedling stage. Genetic analysis based on the inoculation of Bgt isolate E09 on the F-1, F-2, and F-2.3 populations derived from the cross Datoumai x Huixianhong revealed that the powdery mildew resistance of Datoumai is controlled by a single dominant gene, temporarily designated as PmDTM. Bulked segregant analysis and simple sequence repeat mapping with 200 F-2 plants showed that PmDTM was located in the same genetic region as Pm24 on chromosome IDS. To fine map PmDTM, 12 critical recombinants were identified from 1,192 F-2 plants and delimited PmDTM to a 0.5-cM Xhnu58800 to Xhnu59000 interval covering 180.5 Kb (38,728,125 to 38,908,656 bp) on chromosome IDS, and only one highly confident gene, TraesCS1D02G058900, was annotated within this region. TraesCS1D02G058900 encodes a receptor-like serine/threonine-protein kinase (STK), and a 6-bp deletion in exon 5 may confer the resistance to powdery mildew. Allele frequency analysis indicated that the STK allele with 6-bp deletion was only present in three landraces (Datoumai, Chiyacao [Pm24], and Hulutou) and was absent in all of the 353 Chinese modern cultivars and 147 foreign cultivars. These results demonstrate that PmDTM is mapped to the same locus as Pm24 and can be widely used to enhance powdery mildew resistance in wheat growing regions worldwide.
- 相关文献
作者其他论文 更多>>
-
Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew
作者：Fu, Bisheng;Zhang, Qiaofeng;Liu, Caiyun;Cai, Jin;Guo, Wei;Liu, Ying;Zhai, Wenling;Wu, Jizhong;Fu, Bisheng;Liu, Caiyun;Cai, Jin;Guo, Wei;Wu, Jizhong;Fu, Bisheng;Wu, Jizhong;Lin, Zhixin;Xu, Feng;Yan, Lijuan;Gong, Shuangjun
关键词：
Blumeria graminis ; genetic markers; resistance breeding;Triticum aestivum L.;PmHHM -
Genetic diversity and population structure of wheat landraces in Southern Winter Wheat Region of China
作者：Liu, Ying;Fu, Bisheng;Zhang, Qiaofeng;Cai, Jin;Guo, Wei;Zhai, Wenling;Wu, Jizhong;Fu, Bisheng;Cai, Jin;Guo, Wei;Wu, Jizhong;Wu, Jizhong
关键词：Triticum aestivum. L; Landrace; Core collection; Genetic diversity; Population structure
-
Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance
作者：Lu, Chuntian;Du, Jie;Chen, Heyu;Jin, Yinyu;Meng, Xiangru;Zhang, Ting;Xing, Liping;Kong, Lingna;Chen, Peidu;Cao, Aizhong;Zhang, Ruiqi;Lu, Chuntian;Du, Jie;Chen, Heyu;Jin, Yinyu;Meng, Xiangru;Zhang, Ting;Fu, Bisheng;Xing, Liping;Wu, Jizhong;Cao, Aizhong;Zhang, Ruiqi;Gong, Shuangjun;Fu, Bisheng;Wu, Jizhong;Molnar, Istvan;Molnar, Istvan;Holusova, Katerina;Said, Mahmoud;Dolezel, Jaroslav;Said, Mahmoud;Li, Genying
关键词：
-
Quinclorac-resistant Echinochloa spp. promoted growth and reproduction of Laodelphax striatellus (Hemiptera: Delphacidae) probably by providing more nutrients and stable environment
作者：Pi, Yimeng;Xu, Yipeng;Pi, Yimeng;Lu, Yanhui;Mo, Wujia;Li, Qiang;Lu, Zhongxian;Xu, Hongxing;Yang, Xia;Li, Yongfeng
关键词：Herbicide resistance; Echinochloa spp.; Laodelphax striatellus; Ecological fitness
-
Identification and Precise Mapping of PmHSM, a Novel Recessive Powdery Mildew Resistance Allele from Wheat Landrace Heshangmai
作者：Fu, Bisheng;Zhang, Qiaofeng;Cai, Jin;Guo, Wei;Liu, Caiyun;Liu, Ying;Zhai, Wenling;Wu, Jizhong;Fu, Bisheng;Cai, Jin;Guo, Wei;Liu, Caiyun;Wu, Jizhong;Fu, Bisheng;Wu, Jizhong;Gong, Shuangjun
关键词：Blumeria graminis f.sp.tritici; molecular mapping; SSR markers
-
Genetic basis of resistance against powdery mildew in the wheat cultivar "Tabasco"
作者：Wu, Jizhong;Fu, Bisheng;Wu, Jizhong;Jia, Haiyan;Qiao, Linyi;Nagarajan, Ragupathi;Yan, Liuling;Jia, Haiyan;Qiao, Linyi;Brown-Guedira, Gina
关键词：Pm48; Pm2; Powdery mildew; NCF-D-1-1; Wheat
-
Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat
作者：Liu, Caiyun;Guo, Wei;Fu, Bisheng;Liu, Ying;Zhai, Wenling;Wu, Jizhong;Wang, Yang;Zhang, Ruiqi;Dolezel, Jaroslav;Said, Mahmoud;Molnar, Istvan;Holusova, Katerina;Said, Mahmoud;Molnar, Istvan;Molnar, Istvan;Wu, Jizhong;Wu, Jizhong
关键词：Triticum aestivum; Rhizoctonia cerealis; Dasypyrum villosum; Flow sorting; KASP
