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Fine Mapping a Broad-Spectrum Powdery Mildew Resistance Gene in Chinese Landrace Datoumai, PmDTM, and Its Relationship with Pm24

文献类型： 外文期刊

作者： Lu, Nan 1 ; Lu, Mingxue 1 ; Liu, Pan 1 ; Xu, Hongxing 1 ; Qiu, Xiaolong 1 ; Hu, Shanshan 1 ; Wu, Yanan 1 ; Bai, Shenglong 1 ;

作者机构： 1.Henan Univ, Sch Life Sci, State Key Lab Cotton Biol, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Henan, Peoples R China

2.Nanjing Agr Univ, Crop Genom & Bioinformat Ctr, Appl Plant Genom Lab, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Nanjing 210014, Jiangsu, Peoples R China

关键词： Blumeria graminis f. sp. tritici; candidate gene; fine mapping; powdery mildew; Pm24; wheat

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2020 年 104 卷 6 期

页码：

收录情况： SCI

摘要： Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a globally important wheat disease causing severe yield losses, and deployment of resistant varieties is the preferred choice for managing this disease. Chinese wheat landrace Datoumai was resistant to 22 of 23 Bgt isolates at the seedling stage. Genetic analysis based on the inoculation of Bgt isolate E09 on the F-1, F-2, and F-2.3 populations derived from the cross Datoumai x Huixianhong revealed that the powdery mildew resistance of Datoumai is controlled by a single dominant gene, temporarily designated as PmDTM. Bulked segregant analysis and simple sequence repeat mapping with 200 F-2 plants showed that PmDTM was located in the same genetic region as Pm24 on chromosome IDS. To fine map PmDTM, 12 critical recombinants were identified from 1,192 F-2 plants and delimited PmDTM to a 0.5-cM Xhnu58800 to Xhnu59000 interval covering 180.5 Kb (38,728,125 to 38,908,656 bp) on chromosome IDS, and only one highly confident gene, TraesCS1D02G058900, was annotated within this region. TraesCS1D02G058900 encodes a receptor-like serine/threonine-protein kinase (STK), and a 6-bp deletion in exon 5 may confer the resistance to powdery mildew. Allele frequency analysis indicated that the STK allele with 6-bp deletion was only present in three landraces (Datoumai, Chiyacao [Pm24], and Hulutou) and was absent in all of the 353 Chinese modern cultivars and 147 foreign cultivars. These results demonstrate that PmDTM is mapped to the same locus as Pm24 and can be widely used to enhance powdery mildew resistance in wheat growing regions worldwide.

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