文献类型： 外文期刊
作者： Luan, Aiping 1 ; Chen, Chengjie 2 ; Xie, Tao 2 ; He, Junhu 1 ; He, Yehua 2 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Hainan, Peoples R China
2.South China Agr Univ, Coll Hort, Guangzhou 510642, Peoples R China
关键词： pineapple; AcSERK1; embryonic cell-specific promoter; CpG islands; methylation
期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）
ISSN：
年卷期： 2020 年 11 卷 4 期
页码：
收录情况： SCI
摘要： Somatic embryogenesis (SE) is a more rapid and controllable method for plant propagation than traditional breeding methods. However, it often suffers from limited efficiency. SERK1 promotes SE in several plants, including pineapple (Ananas comosus L.). We investigate the embryonic cell-specific transcriptional regulation of AcSERK1 by methylation analysis of CpG islands in AcSERK1 regulatory sequences. This revealed differences in the methylation status of CpG islands between embryonic callus and non-embryonic callus; the methylation inhibitor 5-azaC increased AcSERK1 expression and also accelerated SE. These findings indicate that the expression of AcSERK1 is regulated epigenetically. This study lays the foundation for further analysis of epigenetic regulatory mechanisms that may enhance the efficiency of SE in pineapple and other plants.
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