Engineering Escherichia coli for cost-effective production of medium-chain fatty acids from soy whey using an optimized galactose-based autoinduction system
文献类型： 外文期刊
作者： Wang, Zhe 1 ; Dai, Yiqiang 1 ; Azi, Fidelis 3 ; Wang, Zhongjiang 4 ; Xu, Weimin 1 ; Wang, Daoying 1 ; Dong, Mingsheng 2 ; Xia, Xiudong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China
2.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China
3.Guangdong Technion Israel Inst Technol, Dept Chem Engn, Shantou 515063, Peoples R China
4.Northeast Agr Univ, Coll Food Sci, Harbin 150030, Peoples R China
5.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China
6.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
关键词： Medium-chain fatty acids; Soy whey; Autoinduction system; Escherichia coli; Metabolic engineering
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）
ISSN： 0960-8524
年卷期： 2024 年 393 卷
页码：
收录情况： SCI
摘要： Medium-chain fatty acids (MCFAs) are essential chemical feedstocks. Microbial production of MCFAs offers an attractive alternative to conventional methods, but the costly media and external inducers limit its practical application. To address this issue and make MCFA production more cost-effective, an E.coli platform was developed using soy whey as a medium and galactose as an autoinducer. We first designed an efficient, stringent, homogeneous, and robust galactose-based autoinduction system for the expression of pathway enzymes by rationally engineering the promoter of the galactose-proton symporter (GalP). Subsequently, the intracellular acetyl-CoA availability and NADH regeneration were enhanced to improve the reversal of the beta-oxidation cycle. The resulting strain yielded 8.20 g/L and 16.42 g/L MCFA in pH-controlled batch fermentation and fed-batch fermentation with glucose added using soy whey as medium, respectively. This study provided a cost-effective and promising platform for MCFA production, as well as future strain development for other value-added chemicals production.
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