Molecular cloning, characterization and expression analysis of p53 from turbot Scophthalmus maximus and its response to thermal stress
文献类型： 外文期刊
作者： Huang, Zhihui 1 ; Liu, Xiaofei 1 ; Ma, Aijun 1 ; Wang, Xin-an 1 ; Guo, Xiaoli 1 ; Zhao, Tingting 1 ; Zhang, Jinsheng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Shandong Key Lab Marine Fisheries Biotechnol & Ge, Qingdao 266071, Peoples R China
3.Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
5.Yantai Tianyuan Aquat Ltd Corp, Yantai 264006, Peoples R China
关键词： Sm-p53; Thermal stress; DNA damage; Apoptosis; Turbot Scophthalmus maximus
期刊名称：JOURNAL OF THERMAL BIOLOGY （ 影响因子：2.902； 五年影响因子：3.102 ）
ISSN： 0306-4565
年卷期： 2020 年 90 卷
页码：
收录情况： SCI
摘要： The tumor suppressor protein, p53 plays a crucial role in protecting genetic integrity. Once activated by diverse cell stresses, p53 reversibly activates downstream target genes to regulate cell cycle and apoptosis. However, few studies have investigated the effects of thermal stress in turbot, specifically the p53 signaling pathway. In this study, the rapid amplification of cDNA ends was used to obtain a full-length cDNA of the turbot p53 gene (Sm-p53) and perform bioinformatics analysis. The results showed that the cDNA of the Sm-p53 gene was 2928 bp in length, encoded a 381 amino acid protein, with a theoretical isoelectric point of 6.73. It was composed of a DNA binding and a tetramerization domain. Expression of Sm-p53 in different tissues was detected and quantified by qRT-PCR, and was highest in the liver. We also investigated the expression profiles of Sm-p53 in different tissue and TK cells after thermal stress. These result suggested that Sm-p53 plays a key role, and provides a theoretical basis for Sm-p53 changes in environmental stress responses in the turbot.
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