Effects of Certain cis-Regulatory Elements on Stage-Specific vitellogenin Expression in the Bombyx mori (Lepidoptera: Bombycidae)
文献类型： 外文期刊
作者： Shen, Guanwang 1 ; Liu, Hongling 1 ; Lin, Ying 1 ; Xing, Dongxu 3 ; Zhang, Yujing 1 ; Xia, Qingyou 1 ;
作者机构： 1.Southwest Univ, Biol Sci Res Ctr, Chongqing 400716, Peoples R China
2.Chongqing Key Lab Sericulture Sci, Chongqing 400716, Peoples R China
3.Guangdong Acad Agr Sci, Sericulture & Agrifood Res Inst, Guangzhou 510610, Peoples R China
关键词： vitellogenin; promoter; regulation; transgenic; B. mori
期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）
ISSN：
年卷期： 2020 年 20 卷 3 期
页码：
收录情况： SCI
摘要： Bombyx mori vitellogenin (BmVg) is highly upregulated during pupation, and the 20-hydroxyecdysone and amino acids may regulate stage-specific BmVg expression. However, previous studies showed that other factors may also affect stage-specific BmVg expression. Here, we characterized effective BmVg transcription factors by identifying the corresponding cis-regulatory elements (CREs). We prepared transgenic B. mori, in which DsRed was driven by various lengths of BmVg promoter. qRT-PCR analysis showed that DsRed expression driven by a 1.0-kb BmVg promoter (VgP1.0K) was consistent with endogenous BmVg. VgP1.0K specificity was closer to the endogenous BmVg promoter than that of VgP0.8K. These results suggest that CREs affecting stage-specific BmVg expression were localized to the 1.0-kb BmVg promoter. We investigated the effects of certain CREs that could influence the stage specificity of BmVg promoter on BmVg expression in transgenic B. mori. The relative DsRed expression was significantly reduced in transgenic female B. mori and the peak in DsRed expression was delayed after E-box CRE mutation. These results demonstrate that the E-box element enhanced BmVg expression and also affected stagespecific BmVg expression. Moreover, the relative DsRed expression was significantly increased in transgenic female of B. mori after 3xBD CRE mutation in BmVg promoter. However, the stage specificity of the mutated promoter was consistent with that of the endogenous BmVg promoter. The 3xBD element downregulated BmVg but had no effect on stage-specific BmVg expression.The present study promoted the process of elucidating the regulatory network for stage-specific BmVg expression and furnished a theoretical basis for the application of BmVg promoter.
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