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Detection of aflatoxin B(1)with a new label-free fluorescent aptasensor based on exonuclease I and SYBR Gold

文献类型： 外文期刊

作者： Guo, Zhijun 3 ; Lv, Lei 3 ; Cui, Chengbi 3 ; Wang, Yan 2 ; Ji, Shuang 3 ; Fang, Jiaqi 1 ; Yuan, Ming 5 ; Yu, Hansong 1 ;

作者机构： 1.Jilin Agr Univ, Coll Food Sci & Engn, Changchun 130118, Peoples R China

2.Zhejiang Agr & Forestry Univ, Coll Agr & Food Sci, Hangzhou 310000, Peoples R China

3.Yanbian Univ, Coll Agr, Yanji 133002, Peoples R China

4.Chinese Agr Res Syst, Div Soybean Proc, Soybean Res & Dev Ctr, Changchun 130118, Peoples R China

5.Heilongjiang Acad Agr Sci, Qigihar Branch, Qiqihar 161006, Peoples R China

期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

ISSN： 1759-9660

年卷期： 2020 年 12 卷 22 期

页码：

收录情况： SCI

摘要： This paper describes a new label-free fluorescent aptasensor for the detection of aflatoxin B-1(AFB(1)) based upon exonuclease I (Exo I) and SYBR Gold, in which SYBR Gold, aptamer, AFB(1), and Exo I were used. Specific combinations of aptamer and AFB(1)occurred in the presence of AFB(1)and consequently altered the spatial structure of the aptamer, thereby preventing its digestion by Exo I. When SYBR Gold was added, intense fluorescence was observed. Additionally, a good linear relationship was observed under optimized conditions between the fluorescence intensities and the AFB(1)concentrations (R-2= 0.993). The established aptamer sensor was highly sensitive and exhibited a low limit of detection of 1.82 ng mL(-1), with superior specificity for AFB(1). It was also used in the quantification of AFB(1)levels in soybean sauce samples and demonstrated satisfactory recoveries in the scope of 94.8-108.9%. The proposed sensor is highly sensitive, low cost, and capable of rapid detection and can thus be used to determine mycotoxin levels in a wide range of feeds and food products in a high-throughput and quantitative means.

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